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Correlation between SNP genotypes and periodontitis in Japanese type II diabetic patients: a preliminary study

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Abstract

The present study aims to investigate the correlation between SNP genotype patterns and periodontitis severity in Japanese type II diabetic patients. A cross-sectional study in 43 Japanese diabetic patients with periodontitis was performed. Blood samples were drawn for single nucleotide polymorphism (SNP) analyses and periodontal index (probing pocket depth and clinical attachment level) was subsequently recorded. Twelve functional genes with SNPs that had been shown to be associated with diabetes and/or inflammation were genotyped using a nuclease-mediated SNP-specific ligation method. Subjects with two or more sites with clinical attachment level ≥6 mm and who additionally had one or more sites with pocket depth ≥5 mm were classified as having severe periodontitis. Proportions of risk genotypes/non-risk genotypes between severe and non-severe periodontitis were subsequently compared. A high frequency (21/43 participants, 49 %) of adiponectin gene polymorphism (ADIPOQ 45T > G) homozygous risk genotype (TT genotype) was observed in the participants. The frequency of TGF-β1 SNP (29C > T) risk genotype (TT genotype) in severe periodontitis (34 %, n = 11) was significantly higher than in non-severe periodontitis (0 %, n = 0) (p = 0.04). Our study suggests that TGF-β1 SNPs (29C > T) may be used as one of the risk indicators for severe periodontitis in Japanese diabetic patients.

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Abbreviations

DM:

Diabetes mellitus

IL:

Interleukin

SNP:

Single nucleotide polymorphism

TGF-β1:

Transforming growth factor beta 1

TNF-α:

Tumor necrosis factor alpha

MCP-1:

Monocyte chemotactic protein 1

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Acknowledgments

We would like to thank all study participants for their kind cooperation. Assist. Prof. Dr. Waranuch Pitiphat, Faculty of Dentistry, Khon Kaen University, Thailand for statistical analysis support. This study was supported by Grant-in-Aid from the Japan Society for the Promotion of Science (No. 21792147) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Conflict of interest

The authors declare that they have no conflict of interest in this study.

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Authors and Affiliations

  1. Division of Preventive Dentistry, Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, 2-5274 Gakkocho-Dori, Chuo-Ku, Niigata, 951-8126, Japan

    Teerasak Damrongrungruang, Hiroshi Ogawa, Sayaka Hori-Matsumoto, Kumiko Minagawa & Hideo Miyazaki

  2. Department of Hematology, Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

    Osamu Hanyu & Hirohito Sone

  3. Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand

    Teerasak Damrongrungruang

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  1. Teerasak Damrongrungruang
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  2. Hiroshi Ogawa
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  3. Sayaka Hori-Matsumoto
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  4. Kumiko Minagawa
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  7. Hideo Miyazaki
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Correspondence to Hiroshi Ogawa.

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Damrongrungruang, T., Ogawa, H., Hori-Matsumoto, S. et al. Correlation between SNP genotypes and periodontitis in Japanese type II diabetic patients: a preliminary study. Odontology 103, 233–240 (2015). https://doi.org/10.1007/s10266-014-0171-4

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  • DOI: https://doi.org/10.1007/s10266-014-0171-4

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