Skip to main content

Advertisement

SpringerLink
Log in

Effects of bovine lactoferrin to oral Candida albicans and Candida glabrata isolates recovered from the saliva in elderly people

  • Original Article
  • Published:
Odontology Aims and scope Submit manuscript

Abstract

The effects of bovine lactoferrin (bLF) on the growth of Candida species and on inflammatory cytokine production in gingival keratinocytes, NDUSD-1 co-cultured with Candida strains were investigated. The results showed that bLF at 10 and 100 μg/mL significantly inhibits the growth of two C. albicans strains and two C. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. The levels of inflammatory cytokines, interleukin (IL)-6, and IL-8 in NDUSD-1 co-cultured with each of these four Candida strains were measured. C. albicans tend to have a more potent capacity than C. glabrata to induce the production of the inflammatory cytokines in NDUSD-1. The levels of IL-6 and IL-8 in NDUSD-1 co-cultured with each of Candida species were measured after addition of bLF. bLF at concentrations from 1 to 100 μg/mL significantly inhibited the production of these cytokines in NDUSD-1 co-cultured with Candida species. These findings suggest that bLF may be useful in reducing the risk of aspiration pneumonia among elderly people requiring care for whom oral care is difficult.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Garcia-Montoya IA, Cendon TS, Arevalo-Gallegos S, Rascon-Cruz Q. Lactoferrin a multiple bioactive protein: an overview. Biochim Biophys Acta. 2012;1820:226–36.

    Article  PubMed  Google Scholar 

  2. van der Strate BW, Beljaars L, Molema G, Harmsen MC, Meijer DK. Antiviral activities of lactoferrin. Antiviral Res. 2001;52:225–39.

    Article  PubMed  Google Scholar 

  3. Wang X, Hirmo S, Willen R, Wadstrom T. Inhibition of Helicobacter pylori infection by bovine milk glycoconjugates in a BAlb/cA mouse model. J Med Microbiol. 2001;50:430–5.

    PubMed  Google Scholar 

  4. Ochoa TJ, Brown EL, Guion CE, et al. Effect of lactoferrin on enteroaggregative E. coli (EAEC). Biochem Cell Biol. 2006;84:369–76.

    Article  PubMed  Google Scholar 

  5. Arnold RR, Brewer M, Gauthier JJ. Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms. Infect Immun. 1980;28:893–8.

    PubMed Central  PubMed  Google Scholar 

  6. Wakabayashi H, Yamauchi K, Kobayashi T, et al. Inhibitory effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermedia. Antimicrob Agents Chemother. 2009;53:3308–16.

    Article  PubMed Central  PubMed  Google Scholar 

  7. Takakura N, Wakabayashi H, Ishibashi H, et al. Oral lactoferrin treatment of experimental oral candidiasis in mice. Antimicrob Agents Chemother. 2003;47:2619–23.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Wakabayashi H, Abe S, Teraguchi S, Hayasawa H, Yamaguchi H. Inhibition of hyphal growth of azole-resistant strains of Candida albicans by triazole antifungal agents in the presence of lactoferrin-related compounds. Antimicrob Agents Chemother. 1998;42:1587–91.

    PubMed Central  PubMed  Google Scholar 

  9. Igarashi F, Uchida M, Tsutsui T. Establishment and characterization of cultured human gingival keratinocytes immortalized by transfection of origin (–) SV40 DNA and c-fos gene (in Japanese). Odontology. 1998;86:37–47.

    Google Scholar 

  10. Redding SW, Zellars RC, Kirkpatrick WR, et al. Epidemiology of oropharyngeal Candida colonization and infection in patients receiving radiation for head and neck cancer. J Clin Microbiol. 1999;37:3896–900.

    PubMed Central  PubMed  Google Scholar 

  11. Samaranayake LP, MacFarlane TW. Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin. Arch Oral Biol. 1982;27:869–73.

    Article  PubMed  Google Scholar 

  12. Vermitsky JP, Edlind TD. Azole resistance in Candida glabrata: coordinate upregulation of multidrug. Antimicrob Agents Chemother. 2004;48:3773–81.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Li L, Dongari-Bagtzoglou A. Oral epithelium-Candida glabrata interactions in vitro. Oral Microbiol Immunol. 2007;22:182–7.

    Article  PubMed  Google Scholar 

  14. Kano R, Hasegawa A, Watanabe S, Sato H, Nakamura Y. Candida albicans induced interleukin 8 production by human keratinocytes. J Dermatol Sci. 2003;31:233–5.

    Article  PubMed  Google Scholar 

  15. Schaller M, Mailhammer R, Grassl G, et al. Infection of human oral epithelia with Candida species induces cytokine expression correlated to the degree of virulence. J Invest Dermatol. 2002;118:652–7.

    Article  PubMed  Google Scholar 

  16. Li L, Kashleva H, Dongari-Bagtzoglou A. Cytotoxic and cytokine-inducing properties of Candida glabrata in single and mixed oral infection models. Microb Pathog. 2007;42:138–47.

    Article  PubMed Central  PubMed  Google Scholar 

  17. Bellamy W, Wakabayashi H, et al. Killing of Candida albicans by lactoferricin B, a potent antimicrobial peptide derived from the N-terminal region of bovine lactoferrin. Med Microbiol Immunol. 1993;182:97–105.

    Article  PubMed  Google Scholar 

  18. Okutomi T, Abe S, Tansho S, et al. Augmented inhibition of growth of Candida albicans by neutrophils in the presence of lactoferrin. FEMS Immunol Med Microbiol. 1997;18:105–12.

    Article  PubMed  Google Scholar 

  19. Dongari-Bagtzoglou A, Villar CC, Kashleva H. Candida albicans-infected oral epithelial cells augment the anti-fungal activity of human neutrophils in vitro. Med Mycol. 2005;43:545–9.

    Article  PubMed  Google Scholar 

  20. Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989;84:1045–9.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Rossi D, Zlotnik A. The biology of chemokines and their receptors. Annu Rev Immunol. 2000;18:217–42.

    Article  PubMed  Google Scholar 

  22. Djeu JY, Matsushima K, Oppenheim JJ, Shiotsuki K, Blanchard DK. Functional activation of human neutrophils by recombinant monocyte-derived neutrophil chemotactic factor/IL-8. J Immunol. (Baltimore, Md: 1950) 1990; 144:2205–10.

    Google Scholar 

  23. Jones SA. Directing transition from innate to acquired immunity: defining a role for IL-6. J Immunol. (Baltimore, Md : 1950) 2005; 175:3463–68.

    Google Scholar 

  24. Borish L, Rosenbaum R, Albury L, Clark S. Activation of neutrophils by recombinant interleukin 6. Cell Immunol. 1989;121:280–9.

    Article  PubMed  Google Scholar 

  25. Oram JD, Reiter B. Inhibition of bacteria by lactoferrin and other iron-chelating agents. Biochim Biophys Acta. 1968;170:351–65.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We wish to thank Professor Takeki Tsutsui of the Pharmacology Department at the Nippon Dental University School of Life Dentistry at Tokyo both for his generous technical support and for providing NDUSD-1.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, Japan

    Akino Komatsu & Tazuko Satoh

  2. Morinaga Milk Industry Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa, Japan

    Hiroyuki Wakabayashi

  3. Mitsubishi Chemical Medience Co., 3-30-1 Shimura, Itabashi-ku, Tokyo, Japan

    Fumiaki Ikeda

Authors
  1. Akino Komatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tazuko Satoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroyuki Wakabayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fumiaki Ikeda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akino Komatsu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Komatsu, A., Satoh, T., Wakabayashi, H. et al. Effects of bovine lactoferrin to oral Candida albicans and Candida glabrata isolates recovered from the saliva in elderly people. Odontology 103, 50–55 (2015). https://doi.org/10.1007/s10266-013-0135-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10266-013-0135-0

Keywords

Search

Navigation