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Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells

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Abstract

The cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells such as collagen synthesis and the expression of two osteogenesis-related genes, alkaline phosphatase (ALP) and bone sialoprotein (BSP), was studied using human dental pulp cells (D824 cells). Cellular growth and survival were decreased by treatment of cells with eugenol in a concentration-dependent manner. The incorporation rate of [3H] proline into the acid-insoluble fraction and the synthesis of type I–V collagens were also reduced by treatment of cells with eugenol in a concentration-dependent fashion. The mRNA expression of ALP was scarcely affected in cells exposed to eugenol, whereas the mRNA and protein expression of BSP was down-regulated depending on the concentrations of eugenol. The results suggest that because collagen synthesis and BSP expression play a critical role in hard tissue formation, eugenol used for endodontic treatment may give rise to cytotoxic effects to the normal function of stem cells reported to exist in human dental pulp tissue and periodontal ligament.

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References

  1. Bartelstone HJ. Radioiodine penetration through intact enamel with uptake by blood stream and thyroid gland. J Dent Res. 1951;30:728–33.

    Article  PubMed  Google Scholar 

  2. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97:13625–30.

    Article  PubMed  Google Scholar 

  3. Seo BM, Miura M, Gronthos S, Sartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364:149–55.

    Article  PubMed  Google Scholar 

  4. Chang Y-C, Tai K-W, Huang F-M, Huang M-F. Cytotoxicity and nongenotoxic effects of phenolic compounds in human pulp cell cultures. J Endod. 2000;26:440–3.

    Article  PubMed  Google Scholar 

  5. Gerosa R, Borin M, Menegazzi G, Puttini M, Cavalleri G. In vitro evaluation of the cytotoxicity of pure eugenol. J Endod. 1996;22:532–4.

    Article  PubMed  Google Scholar 

  6. Lin C-P, Chen Y-J, Lee Y-L, Wang J-S, Chang M-C, Lan W-H, Chang H-H, M-W Chao W, Tai T-F, Lee M-Y, Lin B-R, Jeng J-H. Effects of root-end filling materials and eugenol on mitochondrial dehydrogenase activity and cytotoxicity to human periodontal ligament fibroblasts. J Biomed Mater Res Part B: Appl Biomater. 2004;71B:429–40.

    Article  Google Scholar 

  7. Goldberg M, Six M, Decup F, Lasfargues J-J, Salih E, Tompkins K, Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent. 2003;16:66–76.

    PubMed  Google Scholar 

  8. Beertsen W, Vanden Bos T, Everts V. Root development in mice lacking functional tissue non-specific alkaline phosphatase gene: inhibition of acellular cementum formation. J Dent Res. 1999;78:1211–9.

    Google Scholar 

  9. Garcia JMQ, Martins MD, Jaeger RG, Marques MM. Immunolocalization of bone extracellular matrix proteins (type I) collagen, osteonectin and bone sialoprotein in human dental pulp and cultured pulp cells. Int Endod J. 2003;36:404–10.

    Article  PubMed  Google Scholar 

  10. da Silva RAB, Leonardo MR, da Silva LAB, de Castro LMS, Rosa AL, de Oliveria PT. Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro. J Endod. 2008;34:1485–9.

    Article  PubMed  Google Scholar 

  11. Zhang Q, Fan M, Bian Z, Chen Z, Zhu Q. Immunohistochemistry of bone sialoprotein and osteopontin during reparative dentinogenesis in vivo. Chin J Dent Res. 2000;2:38–43.

    Google Scholar 

  12. Tye CE, Hunter GK, Goldberg HA. Identification of the type I collagen-binding domain of bone sialoprotein and characterization of the mechanism of interaction. J Biol Chem. 2005;280:13487–92.

    Article  PubMed  Google Scholar 

  13. Tsutsui TW, Inaba T, Fisher LW, Robey PG, Tsutsui T. In vitro chromosome aberration tests using human dental pulp cells to detect the carcinogenic potential of chemical agents. Odontology. 2006;94:44–50.

    Article  PubMed  Google Scholar 

  14. Kumakura S, Yamamoto A, Yagisawa J, Uchida M, Tsutsui T. Telomere length and telomerase activity in the process of human fibroblast immortalization. Odontology. 2002;90:13–21.

    Article  PubMed  Google Scholar 

  15. Lomri A, Fromique O, Hott M, Marie PJ. Genomic insertion of the SV-40 large T oncogene in normal adult human trabecular osteoblastic cells induces cell growth without less of the differentiated phenotype. Calcif Tissue Int. 1999;64:394–401.

    Article  PubMed  Google Scholar 

  16. Ng SY, Gunning P, Eddy R, Ponte P, Leavitt J, Shows T, Kedes L. Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes. Mol Cell Biol. 1985;5:2720–32.

    PubMed  Google Scholar 

  17. Kubo C, Tsutsui TW, Tamura Y, Kumakura S, Tsutsui T. Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells. Odontology. 2009;97:18–31.

    Article  PubMed  Google Scholar 

  18. Ghosh R, Nadiminty N, Fitzpatrick JE, Alworth WL, Slaga TJ, Kumar AP. Eugenol causes melanoma growth suppression through inhibition of E2F1 transcriptional activity. J Biol Chem. 2005;280:5812–9.

    Article  PubMed  Google Scholar 

  19. Thompson DC, Constantin-Teodosiu D, Moldéus P. Metabolism and cytotoxicity of eugenol in isolated rat hepatocytes. Chem Biol Interact. 1991;77:137–47.

    Article  PubMed  Google Scholar 

  20. Fukuda S. Assessment of the carcinogenic hazard of 6 substances used in dental practices. I. Morphological transformation, DNA damage and sister chromatid exchanges in cultured Syrian hamster embryo cells induced by carbol camphor, eugenol, thymol, EDTA, benzalkonium chloride and benzethonium chloride. Odontology 1987;74:1365–84 (text in Japanese with English abstract).

    Google Scholar 

  21. Hikiba H, Watanabe E, Barrett JC, Tsutsui T. Ability of fourteen chemical agents used in dental practice to induce chromosome aberrations in Syrian hamster embryo cells. J Pharmacol Sci. 2005;97:146–52.

    Article  PubMed  Google Scholar 

  22. Sakano K, Inagaki Y, Oikawa S, Hiraku Y, Kawanishi S. Copper-mediated oxidative DNA damage induced by eugenol: possible involvement of O-demethylation. Mutat Res. 2004;565:35–44.

    PubMed  Google Scholar 

  23. Grant ME, Prockop DJ. The biosynthesis of collagen. N Engl J Med. 1972;286:194–9.

    Article  PubMed  Google Scholar 

  24. Cunningham KW, Lazzari EP, Ranly DM. The effect of formocresol and glutaraldehyde on certain enzymes in bovine dental pulp. Oral Surg Oral Med Oral Pathol. 1982;54:100–3.

    Article  PubMed  Google Scholar 

  25. Hume WR. In vitro studies on the local pharmacodynamics, pharmacology and toxicology of eugenol and zinc oxide-eugenol. Int Endod J. 1988;21:130–4.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan

    Masanori Anpo, Kumiko Shirayama & Takeki Tsutsui

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  1. Masanori Anpo
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  2. Kumiko Shirayama
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  3. Takeki Tsutsui
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Correspondence to Takeki Tsutsui.

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Anpo, M., Shirayama, K. & Tsutsui, T. Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells. Odontology 99, 188–192 (2011). https://doi.org/10.1007/s10266-011-0009-2

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  • DOI: https://doi.org/10.1007/s10266-011-0009-2

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