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Characterization of the cAMP phosphodiesterase domain in plant adenylyl cyclase/cAMP phosphodiesterase CAPE from the liverwort Marchantia polymorpha

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Abstract

Cyclic AMP (cAMP) acts as a second messenger and is involved in the regulation of various physiological responses. Recently, we identified the cAMP-synthesis/hydrolysis enzyme CAPE, which contains the two catalytic domains adenylyl cyclase (AC) and cAMP phosphodiesterase (PDE) from the liverwort Marchantia polymorpha. Here we characterize the PDE domain of M. polymorpha CAPE (MpCAPE-PDE) using the purified protein expressed in E. coli. The Km and Vmax of MpCAPE-PDE were 30 µM and 5.8 nmol min−1 mg−1, respectively. Further, we investigated the effect of divalent cations on PDE activity and found that Ca2+ enhanced PDE activity, suggesting that Ca2+ may be involved in cAMP signaling through the regulation of PDE activity of CAPE. Among the PDE inhibitors tested, only dipyridamole moderately inhibited PDE activity by approximately 40% at high concentrations. Conversely, 3-isobutyl-1-methylxanthine (IBMX) did not inhibit PDE activity.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Number 18K06298 and 21K06236 to M.K.

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  1. Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, 525-8577, Japan

    Yuta Hayashida, Chiaki Yamamoto, Fumio Takahashi, Aika Shibata & Masahiro Kasahara

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  1. Yuta Hayashida
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  2. Chiaki Yamamoto
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  5. Masahiro Kasahara
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Correspondence to Masahiro Kasahara.

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Hayashida, Y., Yamamoto, C., Takahashi, F. et al. Characterization of the cAMP phosphodiesterase domain in plant adenylyl cyclase/cAMP phosphodiesterase CAPE from the liverwort Marchantia polymorpha. J Plant Res 135, 137–144 (2022). https://doi.org/10.1007/s10265-021-01359-4

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