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Empirical measurements of biomechanical anisotropy of the human vocal fold lamina propria

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Abstract

The vocal folds are known to be mechanically anisotropic due to the microstructural arrangement of fibrous proteins such as collagen and elastin in the lamina propria. Even though this has been known for many years, the biomechanical anisotropic properties have rarely been experimentally studied. We propose that an indentation procedure can be used with uniaxial tension in order to obtain an estimate of the biomechanical anisotropy within a single specimen. Experiments were performed on the lamina propria of three male and three female human vocal folds dissected from excised larynges. Two experiments were conducted: each specimen was subjected to cyclic uniaxial tensile loading in the longitudinal (i.e., anterior–posterior) direction, and then to cyclic indentation loading in the transverse (i.e., medial–lateral) direction. The indentation experiment was modeled as contact on a transversely isotropic half-space using the Barnett–Lothe tensors. The longitudinal elastic modulus E L was computed from the tensile test, and the transverse elastic modulus E T and longitudinal shear modulus G L were obtained by inverse analysis of the indentation force-displacement response. It was discovered that the average of E L /E T was 14 for the vocal ligament and 39 for the vocal fold cover specimens. Also, the average of E L /G L , a parameter important for models of phonation, was 28 for the vocal ligament and 54 for the vocal fold cover specimens. These measurements of anisotropy could contribute to more accurate models of fundamental frequency regulation and provide potentially better insights into the mechanics of vocal fold vibration.

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Authors and Affiliations

  1. Mechanical Engineering, Purdue University, 585 Purdue Mall, West Lafayette, IN, 47907, USA

    Jordan E. Kelleher & Thomas Siegmund

  2. Otolaryngology–Head and Neck Surgery, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, 75390, USA

    Mindy Du, Elhum Naseri & Roger W. Chan

  3. Biomedical Engineering, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, 75390, USA

    Roger W. Chan

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  1. Jordan E. Kelleher
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  2. Thomas Siegmund
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  3. Mindy Du
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  4. Elhum Naseri
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  5. Roger W. Chan
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Correspondence to Thomas Siegmund.

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Kelleher, J.E., Siegmund, T., Du, M. et al. Empirical measurements of biomechanical anisotropy of the human vocal fold lamina propria. Biomech Model Mechanobiol 12, 555–567 (2013). https://doi.org/10.1007/s10237-012-0425-4

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