Skip to main content
Log in

A rapid introduction to neurological biochemistry using Drosophila melanogaster

  • Technical Report
  • Published:
Invertebrate Neuroscience

Abstract

Short, cost-effective teaching activities are a useful way of providing an integrated view on biological processes. Here we describe a brief, hands-on workshop that allows pre-university students to explore their understanding of a neurological pathway from its chemical bases to phenotype. The workshop effectively introduces the students to data collection and analysis in an enjoyable way and at an appropriate level, determined by an end of session feedback survey. The design of the workshop can be adapted and scaled to generate diverse sessions such as university teaching practicals or summer school training workshops.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Bellen HJ, Tong C, Tsuda H (2010) 100 years of Drosophila research and its impact on vertebrate neuroscience: a history lesson for the future. Nat Rev Neurosci 11:514–522

    Article  CAS  Google Scholar 

  • Bilder D, Irvine KD (2017) Taking stock of the Drosophila research ecosystem. Genetics 206:1227–1236

    Article  CAS  Google Scholar 

  • Blaschko H (1939) The specific action of l-dopa decarboxylase. J Physiol 96:50P–51P

    Google Scholar 

  • Carpenter JM (1950) A new semisynthetic food medium for Drosophila. Drosoph Inf Serv 24:96–97

    Google Scholar 

  • Corti O, Lesage S, Brice A (2011) What genetics tells us about the causes and mechanisms of Parkinson’s disease. Physiol Rev 91:1161–1218

    Article  CAS  Google Scholar 

  • Daubner SC, Le T, Wang S (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys 508:1–12

    Article  CAS  Google Scholar 

  • Gargano JW, Martin I, Bhandari P, Grotewiel MS (2005) Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila. Exp Gerontol 40:386–395

    Article  Google Scholar 

  • Garris PA, Ciolkowski EL, Pastore P, Wightman RM (1994) Efflux of dopamine from the synaptic cleft in the nucleus accumbens of the rat brain. J Neurosci 14:6084–6093

    Article  CAS  Google Scholar 

  • Hurst WJ, Toomey PB (1981) High-performance liquid chromatographic determination of four biogenic amines in chocolate. Analyst 106:394–402

    Article  CAS  Google Scholar 

  • Intra J, Pasini M (2016) The fruit fly Drosophila as a powerful tool in teaching life sciences in middle and high school classrooms. Int J Innov Res

  • Kalia LV, Lang AE (2015) Parkinson’s disease. Lancet 386:896–912

    Article  CAS  Google Scholar 

  • Kelley P, Whatson T (2013) Making long-term memories in minutes: a spaced learning pattern from memory research in education. Front Hum Neurosci 7:589

    Article  Google Scholar 

  • Lee SB, Kim W, Lee S, Chung J (2007) Loss of LRRK2/PARK8 induces degeneration of dopaminergic neurons in Drosophila. Biochem Biophys Res Commun 358:534–539

    Article  CAS  Google Scholar 

  • Lessing D, Bonini NM (2009) Maintaining the brain: insight into human neurodegeneration from Drosophila melanogaster mutants. Nat Rev Genet 10:359–370

    Article  CAS  Google Scholar 

  • McNicholas S, Potterton E, Wilson KS, Noble MEM (2011) Presenting your structures: the CCP4mg molecular-graphics software. Acta Crystallogr D Biol Crystallogr 67:386–394

    Article  CAS  Google Scholar 

  • Meiser J, Weindl D, Hiller K (2013) Complexity of dopamine metabolism. Cell Commun Signal 11:34

    Article  CAS  Google Scholar 

  • Quintero-Espinosa D, Jimenez-Del-Rio M, Velez-Pardo C (2017) Knockdown transgenic Lrrk Drosophila resists paraquat-induced locomotor impairment and neurodegeneration: a therapeutic strategy for Parkinson’s disease. Brain Res 1657:253–261

    Article  CAS  Google Scholar 

  • Robbins TW (2003) Dopamine and cognition. Curr Opin Neurol 16(Suppl 2):S1–S2

    Article  Google Scholar 

  • Tan S, Hermann B, Borrelli E (2003) Dopaminergic mouse mutants: investigating the roles of the different dopamine receptor subtypes and the dopamine transporter. Int Rev Neurobiol 54:145–197

    Article  CAS  Google Scholar 

  • Wang D, Tang B, Zhao G et al (2008) Dispensable role of Drosophila ortholog of LRRK2 kinase activity in survival of dopaminergic neurons. Mol Neurodegener 3:3

    Article  CAS  Google Scholar 

  • Wang S, Wacker D, Levit A et al (2017) D4 dopamine receptor high-resolution structures enable the discovery of selective agonists. Science 358:381–386

    Article  CAS  Google Scholar 

  • Williams TE (1986) Optimizing student-institution fit. New Directions for Higher Education 1986:35–46

    Article  Google Scholar 

  • Wise RA (2005) Forebrain substrates of reward and motivation. J Comp Neurol 493:115–121

    Article  CAS  Google Scholar 

  • Yorke M, Longden B (2008) The first-year experience of higher education in the UK. Higher Education Academy, York

    Google Scholar 

Download references

Acknowledgements

We would like to thank Dianne Doubtfire and Nicola Charlton for technical support, Stephanie Ellis for administrative support, and Antje Kuhrs for discussions. Chris Elliott and Richard Maguire for fly strains. Natalie Armstrong for suggestion of the development of the workshop. Paul Shields for photography. Jack Munns for the idea of fly racing card. James Chong for critical reading of the manuscript.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Setareh S. Chong.

Ethics declarations

Conflict of interest

None.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chong, S.S., Wilkinson, A.J. & Chawla, S. A rapid introduction to neurological biochemistry using Drosophila melanogaster. Invert Neurosci 18, 15 (2018). https://doi.org/10.1007/s10158-018-0220-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10158-018-0220-8

Keywords

Navigation