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Proliferation, migration, and invasion of human glioma cells exposed to fractionated radiotherapy in vitro

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Abstract

Radiotherapy is a well established treatment for malignant gliomas. This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines (GaMg and U-87 Mg) grown as multicellular tumour spheroids after radiotherapy. Migration and proliferation studies were performed using conventional and accelerated fractionation up to 60 Gy and 59.4 Gy, respectively. A dose-dependent growth and migratory response to irradiation independent of the type of fractionation was observed. A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. Marked invasion of the glioma spheroids into the brain aggregates occurred with or without radiotherapy. For the GaMg cells, flow cytometric DNA histograms after treatment with 10 Gy and 40 Gy showed an accumulation of cells in the G2/M phase of the cell cycle. Radiotherapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.

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Acknowledgements

This work was supported by grants from the Department of Neurosurgery, Lübeck, Germany. The technical assistance of Ms. E. Pawlak is greatly appreciated.

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Authors and Affiliations

  1. Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany

    Jan Gliemroth, Christiane Gerlach, Hans Arnold & A. Jorge A. Terzis

  2. Department of Radiotherapy and Nuclear Medicine, Medical University of Lübeck, Lübeck, Germany

    Thomas Feyerabend

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  1. Jan Gliemroth
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  2. Thomas Feyerabend
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  4. Hans Arnold
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Correspondence to Jan Gliemroth.

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Gliemroth, J., Feyerabend, T., Gerlach, C. et al. Proliferation, migration, and invasion of human glioma cells exposed to fractionated radiotherapy in vitro. Neurosurg Rev 26, 198–205 (2003). https://doi.org/10.1007/s10143-003-0253-1

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  • DOI: https://doi.org/10.1007/s10143-003-0253-1

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