Abstract
A Staphylococcus aureus isolate (SA01) obtained from bloodstream infection exhibited a remarkable drug resistance profile. In this study, we report the draft genome sequence of S. aureus ST 5 SA01, a multidrug-resistant isolate, and analyzed the genes associated with drug resistance and virulence. The genome sketch of S. aureus ST5 SA01 was sequenced with Illumina and annotated using the Prokka software. Rapid Annotation Subsystem Technology (RAST) was used to verify the gene functions in the genome subsystems. The Comprehensive Antibiotic Resistance Database (CARD) and Virulence Factor Database (VFDB) were used in the analysis. The RAST indicated a contribution of 25 proteins to host adenine, fibronectin-binding protein A (FnbA), and biofilm formation as an intercellular polysaccharide adhesive system (PIA). The MLST indicated that S. aureus ST 5 SA01 belongs to ST5 (CC5). In silico analyses also showed an extensive repertoire of genes associated with toxins, such as LukGH leukocidin, enterotoxins, and superantigen staphylococcal classes (SSL). The 11 genes for antimicrobial resistance in S. aureus ST 5 SA01 showed similarity and identity above ≥ 99% with nucleotide sequences deposited in GenBank. Although studies on ST5 clones in Brazil are scarce, monitoring the clone of S. aureus ST 5 SA01 is essential, as it has become a problem in pediatrics in several countries.
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Acknowledgements
The authors thank Hélio Euclides S. dos Santos and Marinaldo for technical assistance.
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This study was funded by Fundação de Amparo à Pesquisa e o Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA, BM-05512/19; INFRA-02032/21; POS-GRAD-02460/21).
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Investigation: Romulo Ferreira, Douglas Santos Silva, Joveliane Monteiro, Gabriella Ferreira, Karinny Silva, Maria Raimunda Silva, and Letícia Oliveira; writing—original draft: Romulo Ferreira; writing—review and editing: Luís Claudio Nascimento da Silva and Andrea Monteiro.
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Our study did not involve human or human tissue samples. The bacterium isolate used in the study was previously isolated as part of other published study and now is part of our Microbiological collection.
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Ferreira, R.M., dos Santos Silva, D.H., Silva, K.F. et al. Draft genome sequence of Staphylococcus aureus sequence type 5 SA01 isolated from bloodstream infection and comparative analysis with reference strains. Funct Integr Genomics 23, 288 (2023). https://doi.org/10.1007/s10142-023-01204-y
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DOI: https://doi.org/10.1007/s10142-023-01204-y