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CircPVT1 facilitates the progression of oral squamous cell carcinoma by regulating miR-143-3p/SLC7A11 axis through MAPK signaling pathway

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Abstract

Oral squamous cell carcinoma (OSCC) is a common malignant tumor occurring in the oral cavity. Circular RNAs (circRNAs) play a crucial regulatory role in many cancers. This study aimed to investigate the function of circRNA plasmacytoma variant translocation 1 (PVT1) (circPVT1) in OSCC and its potential mechanism. The levels of circPVT1, solute carrier family 7 member 11 (SLC7A11), and microRNA-143-3p (miR-143-3p) were examined by quantitative real-time PCR (qRT-PCR) or western blot assay. Cell proliferation, apoptosis, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), colony formation assay, flow cytometry, and transwell assay. The levels of apoptosis and proliferation-related proteins were examined by western blot. The targeting relationship between miR-143-3p and circPVT1 or SLC7A11 was verified by dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA pull-down assays. The levels of mitogen-activated protein kinase (MAPK) pathway-related proteins were measured by western blot. Xenograft assay was used to assess tumor growth in vivo. CircPVT1 and SLC7A11 were upregulated, while miR-143-3p was downregulated in OSCC tissues and cells. Silencing of circPVT1 or SLC7A11 suppressed proliferation, migration, and invasion and promoted apoptosis in OSCC cells. CircPVT1 upregulated SLC7A11 expression via sponging miR-143-3p. SLC7A11 upregulation alleviated the effect of circPVT1 knockdown on OSCC cell progression. Besides, circPVT1 modulated MAPK signaling pathway by regulating miR-143-3p. Moreover, circPVT1 knockdown inhibited tumor growth in vivo. Knockdown of circPVT1 impeded OSCC progression via the miR-143-3p/SLC7A11 axis through MAPK signaling pathway.

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Data availability

The data that support the findings of this study are available on request from the corresponding author.

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Authors

Contributions

All authors contributed to the study conception and design. Clinical data collection, genetic counseling, and follow-up were performed by SW and WL. Data analyses was performed by NL and LY. The methodology was performed by JY and LW. The first draft of the manuscript was written by SW. HZ interpreted the data and provided critical revisions and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hongyu Zhao.

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The design of this protocol follows the tenets of the Declaration of Helsinki, approved by the Ethics Committee of The First Affiliated Hospital of Zhengzhou University.

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The authors declare no competing interests.

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Wang, S., Li, W., Yang, L. et al. CircPVT1 facilitates the progression of oral squamous cell carcinoma by regulating miR-143-3p/SLC7A11 axis through MAPK signaling pathway. Funct Integr Genomics 22, 891–903 (2022). https://doi.org/10.1007/s10142-022-00865-5

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  • DOI: https://doi.org/10.1007/s10142-022-00865-5

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