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Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides

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Abstract

Late blight is a serious economic threat to potato crop, sometimes leading to complete crop loss. The resistance in potato to late blight can be qualitative or quantitative in nature. Qualitative resistance is not durable. Though quantitative resistance is durable, the breeding is challenging due to polygenic inheritance. Several quantitative trait loci (QTLs) have been identified, but the mechanisms of resistance are largely unknown. A nontargeted metabolomics approach was used to identify resistance-related (RR) metabolites in a resistant genotype (F06025), as compared to a susceptible (Shepody) genotype, mock- or pathogen-inoculated. The RR metabolites, which had high fold change in abundance, mainly belonged to phenylpropanoid, flavonoid, fatty acid, and alkaloid chemical groups. The most important phenylpropanoids identified were hydroxycinnamic acid amides, the polyaromatic domain of suberin that is known to be associated with cell wall reinforcement. These metabolites were mapped on to the potato metabolic pathways, and the candidate enzymes and their coding genes were identified. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay revealed a higher upregulation of 4-coumarate: CoA ligase (4-CL), tyrosine decarboxylase (TyDC), and tyramine hydroxycinnamoyl transferase (THT) in the pathogen-inoculated resistant genotype than in susceptible. These genes were sequenced in both resistant and susceptible genotypes, and nonsynonymous single-nucleotide polymorphisms (nsSNPs) were found. The application of these genes in potato resistance improvement, following validation, is discussed.

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Acknowledgments

This work was carried out with the aid of a grant from the International Development Research Center, Ottawa, Canada and with the financial support of the Department of Foreign Affairs, Trade and Development Canada.

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Authors and Affiliations

  1. Plant Science Department, McGill University, Ste.-Anne-de-Bellevue, Quebec, H9X 3V9, Canada

    Kalenahalli N. Yogendra, Doddaraju Pushpa, Kareem A. Mosa & Ajjamada C. Kushalappa

  2. Agriculture and Agri-Food Canada, Fredericton, New Brunswick, Canada

    Agnes Murphy

  3. Department of Agronomy, National University of Colombia, Bogota, Colombia

    Teresa Mosquera

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  1. Kalenahalli N. Yogendra
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  2. Doddaraju Pushpa
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  3. Kareem A. Mosa
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  4. Ajjamada C. Kushalappa
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Correspondence to Ajjamada C. Kushalappa.

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Yogendra, K.N., Pushpa, D., Mosa, K.A. et al. Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides. Funct Integr Genomics 14, 285–298 (2014). https://doi.org/10.1007/s10142-013-0358-8

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