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TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes

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Abstract

Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor complex. Each of the NF-Y subunits (NF-YA, NF-YB and NF-YC) in plants is encoded by multiple genes. Quantitative RT-PCR analysis revealed that five wheat NF-YC members (TaNF-YC5, 8, 9, 11 and 12) were upregulated by light in both the leaf and seedling shoot. Co-expression analysis of Affymetrix wheat genome array datasets revealed that transcript levels of a large number of genes were consistently correlated with those of the TaNF-YC11 and TaNF-YC8 genes in three to four separate Affymetrix array datasets. TaNF-YC11-correlated transcripts were significantly enriched with the Gene Ontology term photosynthesis. Sequence analysis in the promoters of TaNF-YC11-correlated genes revealed the presence of putative NF-Y complex binding sites (CCAAT motifs). Quantitative RT-PCR analysis of a subset of potential TaNF-YC11 target genes showed that ten out of the 13 genes were also light-upregulated in both the leaf and seedling shoot and had significantly correlated expression profiles with TaNF-YC11. The potential target genes for TaNF-YC11 include subunit members from all four thylakoid membrane-bound complexes required for the conversion of solar energy into chemical energy and rate-limiting enzymes in the Calvin cycle. These data indicate that TaNF-YC11 is potentially involved in regulation of photosynthesis-related genes.

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Acknowledgement

This study was supported in part by the Australian Grains Research & Development Corporation.

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Authors and Affiliations

  1. CSIRO Plant Industry, 306 Carmody Road, St. Lucia, QLD, 4067, Australia

    Troy J. Stephenson, C. Lynne McIntyre & Gang-Ping Xue

  2. School of Life Sciences, Queensland University of Technology, G.P.O. Box 2434, Brisbane, QLD, 4001, Australia

    Troy J. Stephenson & Christopher Collet

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  1. Troy J. Stephenson
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  2. C. Lynne McIntyre
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Correspondence to Gang-Ping Xue.

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Stephenson, T.J., McIntyre, C.L., Collet, C. et al. TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes. Funct Integr Genomics 10, 265–276 (2010). https://doi.org/10.1007/s10142-010-0158-3

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