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siRNA Silencing of FpVtg Induces Ovarian Cell Apoptosis in Redtail Prawn, Fenneropenaeus penicillatus

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Abstract

Inadequate gonadal maturation and poor spawning performance increasingly threaten the sustainability of shrimp aquaculture. Unraveling the mechanisms regulating ovarian development and maturation hence is critical to address industry challenges. Vitellogenin (Vtg), a precursor of yolk protein found in the hepatopancreas and ovary of shrimp, plays a key role in facilitating shrimp’s oocyte maturation and embryonic development after oviposition. This study found that FpVtg was specifically expressed in F. penicillatus hepatopancreas and ovary. FpVtg was localized predominantly in the oocyte cytoplasm and distributed uniformly in the hepatopancreas tissue. Silencing FpVtg led to apoptosis in both hepatopancreas and ovary tissues. Furthermore, FpVtg depletion upregulated the expression of ovarian peritrophin 1, ovarian peritrophin 2, serine proteinase inhibitor 6, and juvenile hormone esterase-like carboxylesterase 1, while downregulated that of vitellogenin, delta-9 desaturase, and insulin-like receptor. KEGG pathway analysis implicated such as PI3K-AKT signaling, RNA transport, ECM-receptor interaction, hippo signaling, oocyte meiosis, and apoptosis were enriched and involved in ovarian development. These findings have provided insights into the FpVtg’s reproductive role and the associated regulatory genes and pathways in F. penicillatus. This knowledge can contribute to establishing strategies to improve the breeding and aquaculture production of F. penicillatus by elucidating its vitellogenesis regulation in redtail prawn and other penaeid species. Further characterization of the implicated pathways and genes will clarify the intricacies underlying ovarian maturation.

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Funding

This research was financially supported by a research grant from the National Natural Science Foundation of China (42366006); High-level Talent Scientific Research Start-Up Fund Project of Beibu Gulf University (2022KYQD10); ASEAN Talented Young Scientist Program of Guangxi (ATYSP2022008); Marine Science Program for Guangxi First-Class Discipline, Beibu Gulf University (DRA002, TRA001).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China

    Kianann Tan, Yaxin Dong, Karsoon Tan, Peng Xu & Kit Yue Kwan

  2. Borneo Marine Research Institute, Universiti Malaysia Sabah, Kota Kinabalu City, Sabah, Malaysia

    Leong-Seng Lim

  3. Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus City, 21030, Terengganu, Malaysia

    Khor Waiho

  4. Zhejiang Institute of Freshwater Fisheries, Huzhou City, 313001, China

    Jing Chen

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  1. Kianann Tan
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Contributions

Kianann Tan: methodology, writing — original draft, funding acquisition, conceptualization, resources. Yaxin Dong: investigation, formal analysis. Karsoon Tan: investigation, supervision. Leong-Seng Lim: writing — review and editing, visualization. Khor Waiho: methodology, conceptualization. Jing Chen: formal analysis, visualization. Peng Xu: project administration, supervision, conceptualization. Kit Yue Kwan: funding acquisition, resources, writing — review and editing.

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Correspondence to Peng Xu or Kit Yue Kwan.

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All experiments in this study were conducted under a protocol approved by the Institutional Animal Care and Use Committee (IACUC, China).

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Tan, K., Dong, Y., Tan, K. et al. siRNA Silencing of FpVtg Induces Ovarian Cell Apoptosis in Redtail Prawn, Fenneropenaeus penicillatus. Mar Biotechnol 25, 1176–1190 (2023). https://doi.org/10.1007/s10126-023-10269-6

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