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De Novo Transcriptomic and Life-History Responses of Moina Micrura Under Stress Environment Conditions

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Abstract

Moina micrura represents a promising model species for ecological and ecotoxicological investigations in tropical freshwater ecosystems. Illumina NovaSeq™ 6000 sequencing was employed in this study to analyze M. micrura across three distinct developmental stages: juvenile, adult, and male. Current study successfully annotated 51,547 unigenes (73.11%) derived from seven (7) different databases. A total of 554 genes were found to be significantly upregulated, while 452 genes showed significant downregulation between juvenile and male. Moreover, 1001 genes were upregulated, whereas 830 genes exhibited downregulation between the adult and male. Analysis of differentially expressed genes revealed upregulation of chitin, cuticle, myosin (MYO), mitogen-activated protein kinases (MAPK), fibrillin (FBN), cytochrome (CYP), glutathione s-transferase (GST), vitellogenin (VTG), acetylcholinesterase (AChE), and transforming growth factor beta (TGFB) under unfavorable environmental conditions (male), as compared to favorable environmental conditions (juveniles and adults). These alterations in gene expression significantly impact the phenological and life-history traits of M. micrura. Furthermore, the upregulation of hemoglobin (HMB), doublesex (DSX), juvenile hormone analogs (JHA), heat shock protein (HSP), and methyltransferase (METT) genes in males initiates the sex-switching effects observed in M. micrura. These findings hold substantial value for researchers interested in determining M. micrura sequences for future investigations of gene expression and comparative reproductive genome analysis within the Moina genus and cladoceran families.

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Data Availability

The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.

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Acknowledgements

The authors would like to acknowledge Aquatic Animal Health and Therapeutics Laboratory (AquaHealth), Universiti Putra Malaysia, for permitting the usage of laboratories and equipment. The first author also would like to thank Skim Latihan Akademik Bumiputra (SLAB), awarded by the Ministry of Higher Education (MOHE), Malaysia, for financial support.

Funding

This work was supported by GIST Research Institute (GRI) grant funded by the Gwangju Institute of Science and Technology (GIST) in 2021. Ahmad Zaharin Aris has received research support from GIST.

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Authors and Affiliations

  1. Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Muhammad Raznisyafiq Razak & Ahmad Zaharin Aris

  2. International Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia, Negeri Sembilan, 71050, Port Dickson, Malaysia

    Ahmad Zaharin Aris & Fatimah Md Yusoff

  3. Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Fatimah Md Yusoff

  4. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Zetty Norhana Balia Yusof

  5. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Zetty Norhana Balia Yusof

  6. School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, 123 Cheomdangwagi-Ro, Buk-Gu, Gwangju, 61005, Republic of Korea

    Sang Don Kim & Kyoung Woong Kim

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  1. Muhammad Raznisyafiq Razak
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Contributions

Muhammad Raznisyafiq Razak and Ahmad Zaharin Aris contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Muhammad Raznisyafiq Razak. Ahmad Zaharin Aris contributed to supervision and funding acquisition. The first draft of the manuscript was written by Muhammad Raznisyafiq Razak and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ahmad Zaharin Aris.

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Razak, M.R., Aris, A.Z., Yusoff, F.M. et al. De Novo Transcriptomic and Life-History Responses of Moina Micrura Under Stress Environment Conditions. Mar Biotechnol 25, 473–487 (2023). https://doi.org/10.1007/s10126-023-10220-9

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