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Transcriptome Profiling in the Marine Red Alga Neopyropia yezoensis Under Light/Dark Cycle

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Abstract

Many organisms are subjected to a daily cycle of light and darkness, which significantly influences metabolic and physiological processes. In the present study, Neopyropia yezoensis, one of the major cultivated seaweeds used in “nori,” was harvested in the morning and evening during light/dark treatments to investigate daily changes in gene expression using RNA-sequencing. A high abundance of transcripts in the morning includes the genes associated with carbon–nitrogen assimilations, polyunsaturated fatty acid, and starch synthesis. In contrast, the upregulation of a subset of the genes associated with the pentose phosphate pathway, cell cycle, and DNA replication at evening is necessary for the tight control of light-sensitive processes, such as DNA replication. Additionally, a high abundance of transcripts at dusk encoding asparaginase and glutamate dehydrogenase imply that regulation of asparagine catabolism and tricarboxylic acid cycle possibly contributes to supply nitrogen and carbon, respectively, for growth during the dark. In addition, genes encoding cryptochrome/photolyase family and histone modification proteins were identified as potential key players for regulating diurnal rhythmic genes.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We wish to thank Drs. Katsutoshi Arai and Takafumi Fujimoto (Hokkaido University, Japan) for kindly providing the LightCycler 480 system.

Funding

This work was supported by the Grant-in-Aid for Young Scientists [grant number19K15907 to TU] from the Japan Society for the Promotion of Science.

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  1. Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan

    Sayaka Kominami, Hiroyuki Mizuta & Toshiki Uji

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  1. Sayaka Kominami
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TU was responsible for the design of the experiments and interpretation of the data. SK and TU performed the experiments. SK, TU, and HM wrote the manuscript. All authors have read and approved the final version of the manuscript.

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Correspondence to Toshiki Uji.

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Kominami, S., Mizuta, H. & Uji, T. Transcriptome Profiling in the Marine Red Alga Neopyropia yezoensis Under Light/Dark Cycle. Mar Biotechnol 24, 393–407 (2022). https://doi.org/10.1007/s10126-022-10121-3

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