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The Genome of the Marine Rotifer Brachionus manjavacas: Genome-Wide Identification of 310 G Protein-Coupled Receptor (GPCR) Genes

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Abstract

The marine rotifer Brachionus manjavacas is widely used in ecological, ecotoxicological, and ecophysiological studies. The reference genome of B. manjavacas is a good starting point to uncover the potential molecular mechanisms of responses to various environmental stressors. In this study, we assembled the whole-genome sequence (114.1 Mb total, N50 = 6.36 Mb) of B. manjavacas, consisting of 61 contigs with 18,527 annotated genes. To elucidate the potential ligand–receptor signaling pathways in marine Brachionus rotifers in response to environmental signals, we identified 310 G protein-coupled receptor (GPCR) genes in the B. manjavacas genome after comparing them with three other species, including the minute rotifer Proales similis, Drosophila melanogaster, and humans (Homo sapiens). The 310 full-length GPCR genes were categorized into five distinct classes: A (262), B (26), C (7), F (2), and other (13). Most GPCR gene families showed sporadic evolutionary processes, but some classes were highly conserved between species as shown in the minute rotifer P. similis. Overall, these results provide potential clues for in silico analysis of GPCR-based signaling pathways in the marine rotifer B. manjavacas and will expand our knowledge of ligand–receptor signaling pathways in response to various environmental signals in rotifers.

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Fig. 1

Modified from Kim et al. (2021a)

Fig. 2

Modified from Choi et al. (2021)

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Funding

This work was supported by a grant from the National Research Foundation (2020R1F1A1076854) funded to Jae-Seong Lee and also by a grant of the Collaborative Genome Program of the Korea Institute of Marine Science and Technology Promotion funded by the Ministry of Oceans and Fisheries (No. 20180430).

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  1. Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea

    Duck-Hyun Kim, Eunjin Byeon, Min-Sub Kim, Young Hwan Lee & Jae-Seong Lee

  2. Départment Des Sciences, Université Sainte-Anne, Church Point, NS, B0W 1M0, Canada

    Jun Chul Park

  3. Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki, 852-8521, Japan

    Atsushi Hagiwara

  4. Organization for Marine Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan

    Atsushi Hagiwara

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  1. Duck-Hyun Kim
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Kim, DH., Byeon, E., Kim, MS. et al. The Genome of the Marine Rotifer Brachionus manjavacas: Genome-Wide Identification of 310 G Protein-Coupled Receptor (GPCR) Genes. Mar Biotechnol 24, 226–242 (2022). https://doi.org/10.1007/s10126-022-10102-6

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