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Full-Length Transcriptome of Red Swamp Crayfish Hepatopancreas Reveals Candidate Genes in Hif-1 and Antioxidant Pathways in Response to Hypoxia-Reoxygenation

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Abstract

Red swamp crayfish is particularly prone to exposure to hypoxia-reoxygenation stress on account of the respiration and rhythmic, light-dependent photosynthetic activity of the algae and aquatic grass. Up to now, the regulation mechanisms of the adverse effects of hypoxia-reoxygenation for this species were still unknown, especially the roles of the antioxidant enzymes in reducing oxidative damage during reoxygenation. To screen for vital genes or pathways upon hypoxic-reoxygenation stress, hepatopancreas gene expression profiles were investigated by using a strategy combining second and third generation sequencing. Five groups of samples, including hypoxia for 1 and 6 h with DO of 1.0 mg/L, reoxygenation for 1 and 12 h with DO of 6.8 mg/L, and the samples under normoxia condition, were used for transcriptome sequencing. Twenty Illumina cDNA libraries were prepared to screen for the differentially expressed genes (DEGs) among the 5 groups of samples. Based on the assembled reference full-length transcriptome, 389 and 533 significantly DEGs were identified in the groups under severe hypoxia treatment for 1 and 6 h, respectively. The top three enriched pathways for these DEGs were “protein processing in endoplasmic reticulum,” “MAPK signaling pathway,” and “endocytosis.” Among these DEGs, hypoxia-inducible factor 1α (Hif-1α) and some Hif-1 downstream genes, such as Ugt-1, Egfr, Igfbp-1, Pk, and Hsp70, were significant differentially expressed when exposed to hypoxia stress. A series of antioxidant enzymes, including two types of superoxide dismutase (Cu/ZnSOD and MnSOD), catalase (CAT), and glutathione peroxidase (GPx), were identified to be differentially expressed during hypoxia-reoxygenation treatment, implying their distinct modulation roles on reoxygenation-induced oxidative stress. The full-length transcriptome and the critical genes characterized should contribute to the revelation of intrinsic molecular mechanism being associated with hypoxia/reoxygenation regulation and provide useful foundation for future genetic breeding of the red swamp crayfish.

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Funding

This work was supported in part by the National Key R&D Program of China (no. 2020YFD0900303), the Agricultural Project from Jiangsu Province Science and Technology Agency (no. BE2020348), the Jiangsu Agricultural Science and Technology Innovation Fund (no. CX(20)3005), the earmarked fund for Jiangsu Agricultural Industry Technology System (no. JATS [2021] 409), the China Agriculture Research System (no. CARS-48), and the Agriculture Breeding Project supported by Jiangsu Provincial Department of Agriculture and Rural Affairs (no. PZCZ201746).

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Authors and Affiliations

  1. Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing, 210017, China

    Yu Xu, Hai Lin, Weihui Yan, Jiajia Li, Mengling Sun & Zhiqiang Xu

  2. Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing, China

    Jiaping Chen

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  1. Yu Xu
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  2. Hai Lin
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  3. Weihui Yan
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  4. Jiajia Li
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  5. Mengling Sun
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  7. Zhiqiang Xu
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Correspondence to Jiaping Chen or Zhiqiang Xu.

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Xu, Y., Lin, H., Yan, W. et al. Full-Length Transcriptome of Red Swamp Crayfish Hepatopancreas Reveals Candidate Genes in Hif-1 and Antioxidant Pathways in Response to Hypoxia-Reoxygenation. Mar Biotechnol 24, 55–67 (2022). https://doi.org/10.1007/s10126-021-10086-9

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  • DOI: https://doi.org/10.1007/s10126-021-10086-9

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