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Characterization and Biological Activity of the Ribosomal Protein L10a of the White Shrimp: Fenneropenaeus merguiensis De Man During Vitellogenesis

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Abstract

The molecular events in the ovaries of Fenneropenaeus merguiensis De Man during vitellogenesis were investigated. The ribosomal protein L10a (RPL10a) was characterized and cloned. It consisted of 669 bp and the deduced polypeptide had 217 amino acids (GeneBank™/EBI accession number FJ623402). The calculated molecular mass and pI were 25.7 kDa and 10.06, respectively. An immunohistochemical technique showed that RPL10a was located in the cytoplasm and nucleus of developing oocytes and follicle cells. Treatment of undeveloped ovarian explant cultures with recombinant histidine (His)-RPL10a stimulated the expression of translationally controlled tumor protein (TCTP), heat shock protein 70 (HSP70), and shrimp ovarian peritrophin (SOP) genes, previously shown to be involved in ovarian maturation. The transcripts of all three genes in the ovarian explants showed their highest expression after 4 h incubation with the His-RPL10a at 37°C. The TCTP and HSP70 transcripts declined after 12 h, while the transcript of SOP remained high until 24 h. The His-RPL10a did not stimulate the expression of the TCTP, SOP, and HSP70 genes in shrimp muscle tissue. The information on the molecular behavior of the RPL10a in this study may, in the future, lead to new methods to stimulate ovarian development in shrimp.

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Acknowledgments

We thank Dr. Brian Hodgson, Faculty of Science, Prince of Songkla University for his critical reading of the manuscript and for his valuable comments. This work was supported by the Royal Golden Jubilee Graduate Program from the Thailand Research Fund (TRF) for Monwadee Wonglapsuwan (4.J.PS/48/B.1), Thailand Government Research Fund 2009.

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Authors and Affiliations

  1. Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Monwadee Wonglapsuwan & Wilaiwan Chotigeat

  2. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Wilaiwan Chotigeat

  3. Graduate School of Bioresources, Mie University, Tsu, Mie, Japan

    Teruo Miyazaki

  4. Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand

    Wiriya Loongyai

Authors
  1. Monwadee Wonglapsuwan
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  2. Teruo Miyazaki
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  3. Wiriya Loongyai
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  4. Wilaiwan Chotigeat
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Correspondence to Wilaiwan Chotigeat.

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Wonglapsuwan, M., Miyazaki, T., Loongyai, W. et al. Characterization and Biological Activity of the Ribosomal Protein L10a of the White Shrimp: Fenneropenaeus merguiensis De Man During Vitellogenesis. Mar Biotechnol 12, 230–240 (2010). https://doi.org/10.1007/s10126-009-9220-3

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  • DOI: https://doi.org/10.1007/s10126-009-9220-3

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