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Anti-microfouling Activity of Lipidic Metabolites from the Invasive Brown Alga Sargassum muticum (Yendo) Fensholt

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Abstract

The purification of the chloroform extract from the brown invasive macroalga Sargassum muticum, through a series of chromatographic separations, yielded 12 fractions that were tested against strains of bacteria, microalgae, and fungi involved in marine biofilm formation. The chemical composition of four (a, c, g, and k) out of the six fractions that exhibited anti-microfouling activity was investigated. Fraction a contained saturated and unsaturated linear hydrocarbons (C12–C27). Arachidonic acid was identified as the major metabolite in fraction c whereas fraction g contained mainly palmitic, linolenic, and palmitoleic acids. Fraction k was submitted to further purification yielding the fraction kAcaF1e that was composed of galactoglycerolipids, active against the growth of two of the four bacterial strains (Shewanella putrefaciens and Polaribacter irgensii) and all tested fungi. These promising results, in particular the isolation and the activity of galactoglycerolipids, attest the potential of the huge biomass of S. muticum as a source of new environmentally friendly antifouling compounds.

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Acknowledgments

This research was part of the first author’s Ph.D. project carried out in the Laboratory of Ecophysiology and Biotechnology of Halophytes and Marine algae (LEBHAM, France) under the supervision of the last author, with the financial support of the Brest Metropole Oceane (BMO) and the region Bretagne (PRIR SUBVEMAR). Financial support from the University of Athens, in the form of a “Kapodistrias” grant, is gratefully acknowledged.

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  1. Erwan Plouguerné

    Present address: Laboratório de Produtos Naturais e Ecologia Química Marinha, Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói, 24001-970, Brazil

Authors and Affiliations

  1. Université Européenne de Bretagne, Université de Brest, EA LEBHAM 3877, European Institute for Marine Sciences (IUEM), Place N. Copernic, 29280, Plouzané, France

    Erwan Plouguerné & Valérie Stiger-Pouvreau

  2. Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, 157 71, Athens, Greece

    Efstathia Ioannou, Panagiota Georgantea, Constantinos Vagias & Vassilios Roussis

  3. School of Biological Sciences, University of Portsmouth, Portsmouth, PO1 2DY, England

    Claire Hellio

  4. UMR CNRS 6521, Université de Bretagne Occidentale, CS 3837, Brest, 29238, France

    Edouard Kraffe

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  1. Erwan Plouguerné
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Plouguerné, E., Ioannou, E., Georgantea, P. et al. Anti-microfouling Activity of Lipidic Metabolites from the Invasive Brown Alga Sargassum muticum (Yendo) Fensholt. Mar Biotechnol 12, 52–61 (2010). https://doi.org/10.1007/s10126-009-9199-9

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