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Inhibitory Effect of Deep-sea Water on Differentiation of 3T3-L1 Adipocytes

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Abstract

Currently, the utilization of deep-sea water (DSW) is receiving much attention due to its high productivity, large quantity, and potential for biological application. The 3T3-L1 cell line is a well-established and commonly used in vitro model to assess adipocyte differentiation. Over the course of several days, confluent 3T3-L1 cells can be converted to adipocytes in the presence of an adipogenic cocktail. In this study, the effects of DSW on differentiation adipocyte 3T3-L1 cells were studied. DSW significantly decreased lipid accumulation, a marker of adipogenesis, in a dose-dependent manner. DSW of hardness 1,000 was the most effective for inhibiting adipocyte differentiation without any cytotoxicity. DSW significantly reduced expression mRNA levels of PPARγ and C/EBPα and protein levels of fatty-acid-binding protein and adiponectin. Our results suggest a potential role for DSW as anti-obesity agents by inhibiting adipocyte differentiation mediated through the down-regulated expression of adipogenic transcription factors and adipocyte-specific proteins.

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Acknowledgement

This research was supported by the Korea Institute of Industrial Technology Evaluation and Planning Grant funded by Ministry of Commerce, Industry, and Energy of Korea (2008, Regional Innovation System).

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Authors and Affiliations

  1. Department of Biotechnology, Daegu University, Kyungbuk, Kyungsan, 712-714, South Korea

    Hee Sun Hwang & Jong Won Yun

  2. Marine New Industry Technology Institute, Watervis Co. Ltd., Seoul, 137-070, South Korea

    Seon Hwa Kim, Yung Geun Yoo & Yong Shik Chu

  3. Intractable Disease Research Center, Dongguk University, Gyeongju, 780-714, South Korea

    Yun Hee Shon

  4. Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju, 780-714, South Korea

    Kyung Soo Nam

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  1. Hee Sun Hwang
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Correspondence to Jong Won Yun.

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Hee Sun Hwang and Seon Hwa Kim contributed equally.

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Hwang, H.S., Kim, S.H., Yoo, Y.G. et al. Inhibitory Effect of Deep-sea Water on Differentiation of 3T3-L1 Adipocytes. Mar Biotechnol 11, 161–168 (2009). https://doi.org/10.1007/s10126-008-9131-8

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