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Molecular Detection of Xenostrobus securis and Mytillus Galloprovincialis Larvae in Galician Coast (Spain)

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Abstract

The mussel species Xenostrobus securis from New Zealand was detected in the Spanish coast recently, in the mouth of the Verdugo River into the Vigo Ria. In view of the great importance of the farm mussel sector in this region, the presence of this alien species greatly concerned producers and administration authorities, because of its potential medium- or long-term effects on the autochthonous species, Mytilus galloprovincialis, an important marine resource widely exploited in this location. The goal of this study was to develop a DNA-based technique to identify X. securis and M. galloprovincialis larvae in plankton samples, which would allow monitoring for the presence of X. securis in different points of the Vigo Ria. The techniques used were simplex and multiplex polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and fragment analysis. The application of this system to planktonic samples could be an effective means to assess the presence of the alien species, allowing monitoring if its dispersion is increasing, or on the contrary, if its distribution is restricted to the mouth of the Verdugo River, where X. securis was first detected. In addition, the application of this system at different times could be useful to assess the presence of larvae of these two species in the plankton.

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Acknowledgments

We thank Iain Suthers, University of New South Wales (Australia); Lucila Lares, Department of biological oceanography CICESE (EEUU); Mark Page, University of California (EEUU); Adriana Canapa, Università Politécnica delle Marche (Italy); Jonathan Gellen, Moss Landing Marine Laboratories (EEUU); Mike Hine, Investigation and Diagnostic Centre Bio security (New Zealand); Matthew Harrington, Marine Science Institute University of California (EEUU); Rod Aster, Marine Biology Cawthron (New Zealand); Levent Cavas, Faculty of Arts and Sciences (Turkey); Silvia Franzellitti, Università di Bologna Scienze Ambient (Italy); Shannon Johnson, Monterey Bay Aquarium Research Institute (EEUU); Ottaviani Enzo, Università Degli di Modena e Reggio Emilia (Italy); Renato Bacchetta, Università Degli Studi Di Milano (Italy); Jerome Cachot Université du Havre (France); Charlie Griffiths Marine Biology UCT (South Africa); and others for the collection of samples. We thank Arrate Aldasoro and Nerea Gonzalez-Lavín for their helpful assistance.

This work was funded by Grant PGIDIT05RMA00801CT from Consellería de Innovación e Industria da Xunta de Galicia.

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Correspondence to Francisco J. Santaclara.

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Santaclara, F.J., Espiñeira, M. & Vieites, J.M. Molecular Detection of Xenostrobus securis and Mytillus Galloprovincialis Larvae in Galician Coast (Spain). Mar Biotechnol 9, 722–732 (2007). https://doi.org/10.1007/s10126-007-9023-3

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  • DOI: https://doi.org/10.1007/s10126-007-9023-3

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