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Effects of Cold Shock on Microtubule Organization and Cell Cycle in Gynogenetically Activated Eggs of Olive Flounder (Paralichthys olivaceus)

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Abstract

Cytological changes and subsequent mitotic processes were studied in gynogenetically activated eggs of olive flounder subjected to cold-shock treatment using indirect immunofluorescence staining of isolated blastodisks. Obvious differences between controls and treated eggs were detected during early cell division. The developmental process of haploid control was similar to that of the diploid control except several minutes delayed. Spindles disassembled by the cold-shock treatment regenerated soon after treatment, resulting in the occurrence of the first mitosis. The immature daughter centriole was easily depolymerized by cold-shock treatment, leading to the formation of the bipolar spindle in the first cell cycle and the formation of the monopolar spindle in the second cell cycle, resulting in chromosome set doubling. Some two-cell stage eggs had a monopolar spindle in one blastomere and a bipolar spindle in another during the second mitosis. These eggs had a high potency developing into haploid-diploid mosaics. To the best of our knowledge, this study is the first to clarify the mechanism of chromosome set doubling in marine fishes and provides a preliminary cytological basis for developing a reliable and efficient protocol for mitotic gynogenesis induction by cold-shock treatment in olive flounder.

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References

  • D Bertotto F Cepollaro A Libertini A Barbaro A Francescon P Belvedere J Barbaro L Colombo (2005) ArticleTitleProduction of clonal founders in the European sea bass, Dicentrarchus labrax L., by mitotic gynogenesis Aquaculture 246 115–124 Occurrence Handle10.1016/j.aquaculture.2005.01.004

    Article  Google Scholar 

  • J Castro C Bouza L Sanchez RM Cal F Piferrer P Martinez (2003) ArticleTitleGynogenesis assessment using microsatellite genetic markers in turbot (Scophthalmus maximus) Mar Biotechnol 5 584–592 Occurrence Handle10.1007/s10126-003-0004-x

    Article  Google Scholar 

  • MV Danilchik WC Funk EE Brown K Larkin (1998) ArticleTitleRequirement for microtubules in new membrane formation during cytokinesis of Xenopus eggs Dev Biol 194 47–60 Occurrence Handle10.1006/dbio.1997.8815

    Article  Google Scholar 

  • A Debec (1991) ArticleTitleInactivation fonctionnelle du centrosome de cellules 3T3 de souris par un choc thermique CR Acad Sci III 312 683–687

    Google Scholar 

  • A Debec AM Courgeon M Maingourd C Maisonhaute (1990) ArticleTitleThe response of the centrosome to heat-shock and related stresses in a Drosophila cell line J Cell Sci 96 403–412

    Google Scholar 

  • A Felip G Martinez–Rodriguez F Piferrer M Carrillo S Zanuy (2000) ArticleTitleAFLP analysis confirms exclusive maternal genomic contribution of meiogynogenetic sea bass (Dicentrarchus labrax L.) Mar Biotechnol 2 301–306

    Google Scholar 

  • K Fukasawa (2002) ArticleTitleIntroduction Oncogene 21 6140–6145 Occurrence Handle10.1038/sj.onc.1205771

    Article  Google Scholar 

  • P Galbusera F Volckaert F Ollevier (2000) ArticleTitleGynogenesis in the African catfish Clarias gariepinus (Burchell, 1822). III. Induction of endomitosis and the presence of residual genetic variation Aquaculture 185 25–42 Occurrence Handle10.1016/S0044-8486(99)00339-7

    Article  Google Scholar 

  • DL Gard (1991) ArticleTitleOrganization, nucleation and acetylation of microtubules in Xenopus laevis oocytes: a study by confocal immunofluorescence microscopy Del Biol 143 346–362 Occurrence Handle10.1016/0012-1606(91)90085-H

    Article  Google Scholar 

  • P Harris M Osborn K Weber (1980) ArticleTitleDistribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavage J Cell Biol 84 668–679 Occurrence Handle10.1083/jcb.84.3.668

    Article  Google Scholar 

  • PL Hertzler WH Clark SuffixJr (1993) ArticleTitleThe late events of fertilization in the penaeoidean shrimp Sicyonia ingentis Zygote 1 287–296 Occurrence Handle10.1017/S0967199400001611

    Article  Google Scholar 

  • K Kato R Hayashi D Yuasa S Yamamoto S Miyashita O Murata H Kumai (2002) ArticleTitleProduction of cloned red sea bream, Pagrus major, by chromosome manipulation Aquaculture 207 19–27 Occurrence Handle10.1016/S0044-8486(01)00769-4

    Article  Google Scholar 

  • TD Kocher WJ Lee H Sobolewska D Penman B McAndrew (1998) ArticleTitleA genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus) Genetics 148 1225–1232

    Google Scholar 

  • J Komen ABJ Bongers CJJ Richter WB Muiswinkel Particlevan EA Huisman (1991) ArticleTitleGynogenesis in common carp (Cyprinus carpio): II. The production of homozygous gynogenetic clones and F1 hybrids Aquaculture 92 127–142 Occurrence Handle10.1016/0044-8486(91)90015-Y

    Article  Google Scholar 

  • R Kuriyama GG Borisy (1981) ArticleTitleCentriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy J Cell Biol 91 814–821 Occurrence Handle10.1083/jcb.91.3.814

    Article  Google Scholar 

  • Li Q, Kijima A (2005) Segregation of microsatellite alleles in gynogenetic diploid Pacific abalone (Haliotis discus hannai). Mar Biotechnol [Epub ahead of print]

  • Q Li C Park A Kijima (2002) ArticleTitleIsolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai J Shellfish Res 21 811–815

    Google Scholar 

  • Q Li C Park T Kobayashi A Kijima (2003) ArticleTitleInheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai Mar Biotechnol 5 331–338 Occurrence Handle10.1007/s10126-002-0116-8

    Article  Google Scholar 

  • M Morelli XP Zhu F You PJ Zhang YL Xu Jh Xu (2003) ArticleTitleEffects of heat-shock on cell division and microtubule oganization in zygotes of the shrimp Penaeus indicus (Crustacea, Decapoda) observed with confocal microscopy Aquaculture 216 39–53 Occurrence Handle10.1016/S0044-8486(01)00894-8

    Article  Google Scholar 

  • K Naruse K Ijiri A Shima N Egami (1985) ArticleTitleThe production of cloned fish in Medaka (Oryzias latipes) J Exp Zool 236 335–341 Occurrence Handle10.1002/jez.1402360311

    Article  Google Scholar 

  • KF O'Connell (2002) ArticleTitleThe ZYG-1 kinase, a mitotic and meiotic regulator of centriole replication Oncogene 21 6201–6208 Occurrence Handle10.1038/sj.onc.1205776

    Article  Google Scholar 

  • I Paschos L Natsis C Nathanailides I Kagalou E Kolettas (2001) ArticleTitleInduction of gynogenesis and androgenesis in goldfish Carassius auratus (var. oranda) Reprod Domest Anim 36 8–19 Occurrence Handle10.1046/j.1439-0531.2001.00285.x

    Article  Google Scholar 

  • S Peruzzi B Chatain (2000) ArticleTitlePressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability Aquaculture 189 23–37 Occurrence Handle10.1016/S0044-8486(00)00355-0

    Article  Google Scholar 

  • A Rousselet U Euteneuer N Bordes T Ruiz G Hui Bon Hua M Bornens (2001) ArticleTitleStructural and functional effects of hydrostatic pressure on centrosomes from vertebrate cells Cell Motil Cytoskelet 48 262–276 Occurrence Handle10.1002/cm.1014

    Article  Google Scholar 

  • G Streisinger C Walker N Dower D Knauber F Singer (1981) ArticleTitleProduction of clones of homozygous diploid zebrafish (Brachydanio rerio) Nature 291 293–296 Occurrence Handle10.1038/291293a0

    Article  Google Scholar 

  • PM Wasserman K Fujiwara (1978) ArticleTitleImmunofluorescent anti-tubulin staining of spindles during meiotic maturation of mouse oocytes in vitro J Cell Sci 29 171–188

    Google Scholar 

  • E Yamamoto (1999) ArticleTitleStudies on sex-manipulation and production of cloned populations in hirame, Paralichthys olivaceus (Temminck et Schlegel) Aquaculture 173 235–246 Occurrence Handle10.1016/S0044-8486(98)00448-7

    Article  Google Scholar 

  • WP Young PA Wheeler VH Coryell P Keim GH Thorgaard (1998) ArticleTitleA detailed linkage map of rainbow trout produced using doubled haploids Genetics 148 839–850

    Google Scholar 

  • XL Zhang H Onozato (2004) ArticleTitleHydrostatic pressure treatment during the first mitosis does not suppress the first cleavage but the second one Aquaculture 240 101–113 Occurrence Handle10.1016/j.aquaculture.2004.07.004

    Article  Google Scholar 

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30271036), the National 863 Program of China (2002AA629160), and the Shandong Natural Science Foundation of China.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People's Republic of China

    Xiang-Ping Zhu, Feng You, Pei-Jun Zhang, Yong-Li Xu & Jian-He Xu

  2. Department of Animal Science, Laiyang Agricultural University, Laiyang, 265200, People's Republic of China

    Xiang-Ping Zhu

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  1. Xiang-Ping Zhu
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  2. Feng You
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  4. Yong-Li Xu
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Correspondence to Feng You or Pei-Jun Zhang.

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Zhu, XP., You, F., Zhang, PJ. et al. Effects of Cold Shock on Microtubule Organization and Cell Cycle in Gynogenetically Activated Eggs of Olive Flounder (Paralichthys olivaceus). Mar Biotechnol 8, 312–318 (2006). https://doi.org/10.1007/s10126-006-5128-3

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  • DOI: https://doi.org/10.1007/s10126-006-5128-3

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