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Exocellular Cyclic Dipeptides from a Ruegeria Strain Associated with Cell Cultures of Suberites domuncula

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Abstract

From cell cultures of Suberites domuncula was isolated a bacterial strain, SDC-1, which was identified by 16S ribosomal RNA sequence analysis as an α-Proteobacterium of the genus Ruegeria. The occurrence of the strain in sponge cell culture could be explained by its resistance to the antibiotics used in the isolation of sponge cell cultures or by the preservation of SDC-1 by host sponge cells. The fatty acid composition of SDC-1 is characterized by branched C-12 methyl fatty acids. Two new and 8 known cyclic dipeptides were isolated and characterized from the fermentation broth of SDC-1. Cyclodipeptides are one of the families of cell-cell signaling compounds and may have some role to play in sponge-bacteria interactions.

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Acknowledgements

This research was supported by a Marie Curie fellowship of the European Community program “Quality of Life and Management of Living Resources” contract QLK5-CT-2001-50974 and by Project SPONGE, contract QLK3-1999-00672. “Servizio di Spettrometria di Massa del CNR-Napoli and Servizio NMR, ICB-CNR” provided the MS and NMR spectra, respectively. The assistance of Dr. S. De Caro, Mr. C. Iodice, and O. De Luca is gratefully acknowledged.

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Authors and Affiliations

  1. Istituto di Chimica Biomolecole CNR, via Campi Flegrei, 34, I-80078 Pozzuoli (Napoli), Italy

    Maya Mitova, Giuseppina Tommonaro & Salvatore De Rosa

  2. Institut für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany

    Ute Hentschel

  3. Institute für Physiologische Chemie, Angewandet Molekularbiologie, Universitat, Duesbergweg 6, D-55099 Mainz, Germany

    Werner E. G. Müller

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  1. Maya Mitova
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  2. Giuseppina Tommonaro
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  4. Werner E. G. Müller
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  5. Salvatore De Rosa
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Correspondence to Salvatore De Rosa.

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Mitova, M., Tommonaro, G., Hentschel, U. et al. Exocellular Cyclic Dipeptides from a Ruegeria Strain Associated with Cell Cultures of Suberites domuncula . Mar. Biotechnol. 6, 95–103 (2004). https://doi.org/10.1007/s10126-003-0018-4

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