Abstract
A Gram-negative, aerobic bacterial strain RR6T was isolated from the sea sand to produce lipase and proposed as a novel species of Halopseudomonas. The optimum growth occurred at 28–37 °C, and the pH was 6.0–8.0. The optimum growth occurred at 3.0 -6.5% (w/v) NaCl. The major cellular fatty acids were C10:0 3OH, C12:0, C16:1 ω7c/16:1 ω6c, 18:1 ω7c and/or 18:1 ω6c, and C16:0. The predominant polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, unidentified phospholipid, and unidentified lipids. The genome is 3.93 Mb, and the G + C content is 61.3%. The 16S rRNA gene sequences shared 99.73–99.87% sequence similarity with the closely related type strains of Halopseudomonas. The average nucleotide identity and average amino acid identity of strain RR6T with reference type strains were below 95–96%, and the corresponding in-silico DNA–DNA hybridization values were below 70%. Strain RR6T clustered with Halopseudomonas gallaeciensis V113T and Halopseudomonas pachastrellae CCUG 46540 T in the phylogenetic tree. Further, lipase produced by this bacterium belongs to α/β hydrolase lipase family and exhibits structural similarity to the lactonizing lipase. Based on the polyphasic analysis, the new isolates RR6T represent a novel species of Halopseudomonas for which Halopseudomonas maritima sp. nov. is proposed. The type strain is RR6T (= NBRC 115418 T = TBRC 15628 T).
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Data availability
The GenBank/EMBL/DDBJ accession numbers for the whole genome and 16S rRNA sequences of Halopseudomonas maritima strain RR6T are MZ855216 and CP079801, respectively.
Abbreviations
- ANI:
-
Average nucleotide identity
- AAI:
-
Average amino acid identity
- DDH:
-
DNA–DNA hybridization
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Acknowledgements
The author R.R.A.K. acknowledge the Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India for providing the research fellowship. We acknowledge the Distributed Information Sub-Center (DISC) at the Institute of Life Sciences, Bhubaneswar, for the computational facility and Transmission Electron Microscopic Facility. We are thankful to Professor B. Schink (Universitaet Konstanz, Germany) for etymological advice.
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This work was supported by the funding received from the Department of Biotechnology, Government of India (D.O.No. BT/BI/04/058/2002 VOL-II) to SKD.
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SKD: developed the concept and designed the experiment. RRAK, MG, SB conducted the experiment. RRAK, MG, SB and SKD analysed the data and wrote the manuscript. All of us read and approved the final manuscript.
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Kujur, R.R.A., Ghosh, M., Basak, S. et al. Phylogeny and structural insights of lipase from Halopseudomonas maritima sp. nov., isolated from sea sand. Int Microbiol 26, 1021–1031 (2023). https://doi.org/10.1007/s10123-023-00362-0
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DOI: https://doi.org/10.1007/s10123-023-00362-0