Skip to main content
SpringerLink
Log in

Effects of photobiomodulation in the experimental acetic acid–induced colitis: comparison between male and female

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Ulcerative colitis (UC) is an important chronic and multifactorial disease, which alters the colon mucosal with a significant impact on life quality affecting both men and women. The difference between genders causes changes in the inflammatory processes, modulating the development of several diseases. The available drugs to treat UC exhibit limited outcomes and side effects; thus, new therapies are needed. Photobiomodulation (PBM) emerges as potential treatment by modulating the inflammatory process without side effects and low costs. The aim of this study was to evaluate the effects of PBM in acetic acid-induced UC comparing the responses between male and females. For this purpose, male and female Wistar rats (36) were submitted to induction of UC by rectal administration of 10% acetic acid (colitis group) and treated or not with PBM (colitis-PBM group) (LED, 660 nm, 100 mW, 150 s) in three points: right side and left of the ventral surface and in the external anal region. Non-manipulated rats were used as control (basal group). We investigated the disease activity index (DAI score), myeloperoxidase enzyme activity (MPO) and release of cytokines in the intestine homogenates, and histological analysis. PBM reduces DAI score, MPO activity, and mast cell degranulation while increased mucous production in both females and males. Moreover, PBM reduced histopathological score as well as the levels of IL-6 and IL-4 in the bowel only in males. We also showed reduced levels of IL-1beta and TNF-alpha after PBM in both males and females, while the levels of IL-10 and IFN-gamma were increased. In conclusion, despite our study has shown some differences between males and females, PBM attenuated the biomarkers of UC in both genders constituting a potential combined treatment that is non-invasive and low cost.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Wilhelm SM, Love BL (2017) Management of patients with inflammatory bowel disease: current and future treatments. Clin Pharm 9(3):1–24. https://doi.org/10.1211/CP.2017.20202316

    Article  Google Scholar 

  2. Khorshidi M, Djafarian K, Aghayei E, Shab-Bidar S (2020) A posteriori dietary patterns and risk of inflammatory bowel disease: a meta-analysis of observational studies. Int J Vitam Nutr Res 90(3–4):376–384. https://doi.org/10.1024/0300-9831/a000508

    Article  CAS  PubMed  Google Scholar 

  3. Romberg-Camps MJ, Dagnelie PC, Kester AD, Hesselink-van de Kruijs MA, Cilissen M, Engels LG, Van Deursen C, Hameeteman WH, Wolters FL, Russel MG, Stockbrügger RW (2009) Influence of phenotype at diagnosis and of other potential prognostic factors on the course of inflammatory bowel disease. Am J Gastroenterol 104(2):371–83. https://doi.org/10.1038/ajg.2008.38

    Article  CAS  PubMed  Google Scholar 

  4. Ghosh S, Mitchell R (2007) Impact of inflammatory bowel disease on quality of life: results of the European Federation of Crohn’s and Ulcerative Colitis Associations (EFCCA) patient survey. J Crohns Colitis 1(1):10–20. https://doi.org/10.1016/j.crohns.2007.06.005

    Article  PubMed  Google Scholar 

  5. Luissint AC, Parkos CA, Nusrat A (2016) Inflammation and the intestinal barrier: leukocyte-epithelial cell interactions, cell junction remodeling, and mucosal repair. Gastroenterology. 151(4):616–32. https://doi.org/10.1053/j.gastro.2016.07.008

    Article  CAS  PubMed  Google Scholar 

  6. Cavalcante RMS, Moura MSB de, Braz DC, Nogueira N do N. Ulcerative colitis and cytokines: a literature review. RSD [Internet]. 2020 Aug. 20 [cited on 2023 Apr.17];9(9):e314997145. Available at: https://rsdjournal.org/index.php/rsd/article/view/7145

  7. Paula-Silva M, Broering MF, Scharf P, da Rocha GHO, Farsky S, Lino-Dos-Santos-Franco A (2020) Red light-emitting diode treatment improves tissue recovery in DSS-induced colitis in mice. J Photochem Photobiol B 212:112018. https://doi.org/10.1016/j.jphotobiol.2020.112018

  8. Kaplan GG, Bernstein CN, Coward S, Bitton A, Murthy SK, Nguyen GC, Lee K, Cooke-Lauder J, Benchimol EI (2019) The impact of inflammatory bowel disease in Canada 2018: epidemiology. J Can Assoc Gastroenterol. 2(Suppl 1):S6–S16. https://doi.org/10.1093/jcag/gwy054

    Article  PubMed  Google Scholar 

  9. Scribano ML, Prantera C (2013) Antibiotics and inflammatory bowel diseases. Dig Dis 31(3–4):379–384. https://doi.org/10.1159/000354704

    Article  PubMed  Google Scholar 

  10. Klein SL, Flanagan KL (2016) Sex differences in immune responses. Nat Rev Immunol 16(10):626–638. https://doi.org/10.1038/nri.2016.90

    Article  CAS  PubMed  Google Scholar 

  11. Bonnegarde-Bernard A, Jee J, Fial MJ, Steiner H, DiBartola S, Davis IC, Cormet-Boyaka E, Tomé D, Boyaka PN (2014) Routes of allergic sensitization and myeloid cell IKKβ differentially regulate antibody responses and allergic airway inflammation in male and female mice. PLoS ONE 9(3):e92307. https://doi.org/10.1371/journal.pone.0092307

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Jaillon S, Berthenet K, Garlanda C (2019) Sexual dimorphism in innate immunity. Clin Rev Allergy Immunol 56(3):308–321. https://doi.org/10.1007/s12016-017-8648-x

    Article  CAS  PubMed  Google Scholar 

  13. Lisboa LA, Santos DC dos, Oliveira C da C, Moura IGS, Bastos LP, Leite TO. Inflammatory response and sexual dimorphism to an infection. Braz. J. Hea. Rev. [Internet]. 26 de outubro de 2021 [citado em 17 de abril de 2023];4(5):23047–56. Disponívelem: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/38290

  14. Owusu G, Obiri DD, Ainooson GK, Osafo N, Antwi AO, Duduyemi BM, Ansah C (2020) Acetic acid-induced ulcerative colitis in sprague dawley rats is suppressed by hydroethanolic extract of Cordia vignei leaves through reduced serum levels of TNF-α and IL-6. Int J Chronic Dis 6(2020):8785497. https://doi.org/10.1155/2020/8785497

    Article  Google Scholar 

  15. Carvalho L do C, Silva ES da, Roma ALM, Lauriano JEG, Reis SC dos, Costa FVS, Bezerra RO de C, Rocha MFQ, Filho VQM, Machado LC de S. Inflammatory bowel diseases: an overview. REAMed [Internet]. 2 Feb.2022 [quoted 17 Apr.2023];2:e9650. Available from: https://acervomais.com.br/index.php/medico/article/view/9650

  16. Belém MO, de Andrade GMM, Carlos TM, Guazelli CFS, Fattori V, Toginho Filho DO, Dias IFL, Verri WA Jr, Araújo EJA (2016) Light-emitting diodes at 940nm attenuate colitis-induced inflammatory process in mice. J Photochem Photobiol B 162:367–373. https://doi.org/10.1016/j.jphotobiol.2016.07.008

    Article  CAS  PubMed  Google Scholar 

  17. Luo J, Cao J, Jiang X, Cui H (2010) Effect of low molecular weight heparin rectal suppository on experimental ulcerative colitis in mice. Biomed Pharmacother 64(7):441–445. https://doi.org/10.1016/j.biopha.2010.01.013

    Article  CAS  PubMed  Google Scholar 

  18. Kiesler P, Fuss IJ, Strober W (2015) Experimental models of inflammatory bowel diseases. Cell Mol Gastroenterol Hepatol 1(2):154–170. https://doi.org/10.1016/j.jcmgh.2015.01.006

    Article  PubMed  PubMed Central  Google Scholar 

  19. Kooijman EE, Kuzenko SR, Gong D, Best MD, Folkesson HG (2011) Phosphatidylinositol 4,5-bisphosphate stimulates alveolar epithelial fluid clearance in male and female adult rats. Am J Physiol Lung Cell Mol Physiol 301(5):L804–L811. https://doi.org/10.1152/ajplung.00445.2010

    Article  CAS  PubMed  Google Scholar 

  20. Angele MK, Pratschke S, Chaudry IH (2012) Does gender influence outcomes in critically ill patients? Crit Care 16(3):129. https://doi.org/10.1186/CC11365

    Article  PubMed  PubMed Central  Google Scholar 

  21. Rodrigues FAP de, Medeiros PHQSde, Prata MMG de, Lima AÂM (2016) Physiology of the intestinal epithelial barrier, p 441–478. In: Digestive System: Basic-Clinical Integration. So Paulo: Blucher

  22. Ji Y, Sakata Y, Yang Q, Li X, Xu M, Yoder S, Langhans W, Tso P (2012) Activation of rat intestinal mucosal mast cells by fat absorption. Am J Physiol Gastrointest Liver Physiol. 302(11):G1292-300. https://doi.org/10.1152/ajpgi.00011.2012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Lopes Silva JC, Campos S Évelin DC, Santana MLC de, Costa A dos S, Francelino JO. Gut microbiota and the central nervous system: exploring the brain-gut axis. Rev Neuroscience [Internet]. April 25, 2022 [quoted April 17, 2023]; 30:1–29. Available at: https://periodicos.unifesp.br/index.php/neurociencias/article/view/12129

  24. Schmitt N, Ueno H (2015) Regulation of human helper T cell subset differentiation by cytokines. Curr Opin Immunol. 34:130–6. https://doi.org/10.1016/j.coi.2015.03.007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This study was sponsored by CNPq 302746/2020–0.

Author information

Authors and Affiliations

  1. Post Graduate Program in Biophotonic Medicine, University Nove de Julho (UNINOVE), Rua Vergueiro, 239/245, São Paulo, SP CEP, 01504-000, Brazil

    Kelly Cristina Quintela Martins Bitencourt, Adriana Schapochnik, Vanessa de Souza, Virgínia Mendes Matias Rodrigues, Marlon Palma da Cruz, Rebeca Boltes Cecatto, Maria Fernanda Setúbal Destro Rodrigues & Adriana Lino-dos-Santos-Franco

  2. Department of Basic Science in Health, Faculty of Medical Sciences, Federal University of Cuiabá, Cuiabá, Brazil

    Amílcar Sabino Damazo

  3. Department of Biological Sciences, University Federal de São Paulo (UNIFESP), Diadema, Brazil

    Caroline Marcantonio Ferreira

Authors
  1. Kelly Cristina Quintela Martins Bitencourt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adriana Schapochnik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vanessa de Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Virgínia Mendes Matias Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marlon Palma da Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Amílcar Sabino Damazo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Caroline Marcantonio Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Rebeca Boltes Cecatto
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Maria Fernanda Setúbal Destro Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Adriana Lino-dos-Santos-Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adriana Lino-dos-Santos-Franco.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bitencourt, K.C.Q.M., Schapochnik, A., de Souza, V. et al. Effects of photobiomodulation in the experimental acetic acid–induced colitis: comparison between male and female. Lasers Med Sci 38, 271 (2023). https://doi.org/10.1007/s10103-023-03932-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10103-023-03932-w

Keywords

Search

Navigation