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Determination of cadmium induced acute and chronic reproductive toxicity with Raman spectroscopy

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Abstract

Cadmium (Cd) is one of the toxic heavy metals which is confirmed to be related to male sterile. Here, confocal Raman spectroscopy was employed to detect biomolecular composition and changes in testis under acute and chronic Cd treatment. Specific Raman shifts associated with mitochondria, nucleic acids, proteins, lipids, and cholesterol were identified which were distinguishing among groups undergoing different Cd treatment times. Supporting evidences were provided by conventional experimental detections. The relevant biochemical parameters, pathological changes, and protein expression related to testosterone synthesis were all changed and consistent with Raman spectrum information. In conclusion, confocal Raman spectroscopy presents a reliable data and provides a novel method which is expected to be a promising strategy in reproduction toxicity research.

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Authors and Affiliations

  1. Department of Urologic Surgery, The First Hospital of Lanzhou University, Lanzhou, 730000, Gansu, China

    Disheng Liu & Jianghou Wan

  2. Department of Anaesthesiology, The First Hospital of Lanzhou University, Lanzhou, 730000, Gansu, China

    Disheng Liu, Zhenzhen Liu, Zhiyu Zhao, Guangru Zhang & Yufang Leng

Authors
  1. Disheng Liu
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  2. Jianghou Wan
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  3. Zhenzhen Liu
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  4. Zhiyu Zhao
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  5. Guangru Zhang
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  6. Yufang Leng
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Correspondence to Yufang Leng.

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The research was approved by the Ethics Committee of Lanzhou University (permit number SCXK Gan 2013-0002) and all the efforts were made to minimize the animal suffering

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The authors declare that they have no conflicts of interest.

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Liu, D., Wan, J., Liu, Z. et al. Determination of cadmium induced acute and chronic reproductive toxicity with Raman spectroscopy. Lasers Med Sci 35, 1919–1926 (2020). https://doi.org/10.1007/s10103-020-02976-6

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  • DOI: https://doi.org/10.1007/s10103-020-02976-6

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