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Influence of Er:YAG laser pulse duration on the long-term stability of organic matrix and resin-dentin interface

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Abstract

The purpose of this study was to explore the influence of Er:YAG laser irradiation with different pulse durations on the organic matrix, micromorphology of the hybrid layer (HL), and bond strength over time. Sixty caries-free human molars were cut to obtain flat dentin surfaces which were randomly divided into 4 groups: control (not irradiated–G1) and laser groups (80 mJ/2 Hz) with pulse duration ranging between 50 (G2), 300 (G3), and 600 μs (G4). A self-etch adhesive system (Universal 3M ESPE) was applied on pre-treated dentin surfaces and cylinders of resin composite were built up and stressed in a universal testing machine (μSBS) at 24 h and after12 months (n = 12). In addition, 3 other dentin-bonded specimens were prepared as previously described for each group with the adhesive doped with 0.1 wt% Rhodamine B to analyze hybrid layer morphology under Confocal Laser Microscope Scanning (CLMS). Organic matrix and collagen fibrils were analyzed by second harmonic generation (SGH). Two-way ANOVA and Tukey’s test detected significantly higher μSBS values for the control group, whereas the lower values were observed in all laser groups at 24 h (p < 0.05). Storage in artificial saliva did not reduce μSBS in all groups. The low signal emitted by SHG images below the irradiated area demonstrated thermal damage of the collagen matrix. CLMS images of laser groups exhibited thicker and irregular resin-dentin interfaces than the control group. Regardless of the pulse duration, Er:YAG laser pre-treatment altered the organic matrix and HL formation which resulted in low μSBS values at 24 h. The alterations on dentin’s organic structure did not jeopardize the μSBS after 1 year of saliva storage.

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Acknowledgments

The authors would like to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the financial support given for the purchase of the laser equipment (Fidelis III-Fotona), Grant # 2015/12651-1.

Funding

This study was funded by FAPESP (2015/12651-1 and 2015/13571-1).

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Authors and Affiliations

  1. Department of Operative Dentistry, School of Dentistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil

    Livia Tosi Trevelin, Beatriz Togoro Ferreira da Silva, Patrícia Moreira de Freitas & Adriana Bona Matos

  2. Department of Operative Dentistry, School of Dentistry, University of Cruzeiro do Sul, Av. Rua Galvão Bueno, 868, São Paulo, SP, 01506-000, Brazil

    Livia Tosi Trevelin

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  1. Livia Tosi Trevelin
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  2. Beatriz Togoro Ferreira da Silva
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Correspondence to Livia Tosi Trevelin.

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Ethics approval

Human third molars used in this in vitro study followed the approval of the Human Research Ethics Committee of the School of Dentistry of the University of São Paulo, Brazil (Protocol n. 124143/2015).

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Trevelin, L.T., da Silva, B.T.F., de Freitas, P.M. et al. Influence of Er:YAG laser pulse duration on the long-term stability of organic matrix and resin-dentin interface. Lasers Med Sci 34, 1391–1399 (2019). https://doi.org/10.1007/s10103-019-02739-y

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  • DOI: https://doi.org/10.1007/s10103-019-02739-y

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