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Low-level laser therapy as an alternative for pulpotomy in human primary teeth

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Abstract

This study aimed to evaluate the effects of low-level laser therapy (LLLT) on pulpal response of primary teeth. Twenty mandibular primary molars were randomly divided into the following groups: group I Buckley’s formocresol (diluted at 1:5), group II calcium hydroxide, group III LLLT + zinc oxide/eugenol, and group IV LLLT + calcium hydroxide. LLLT parameters were set at 660-nm wavelength, 10-mW power output, and 2.5 J/cm2 energy density for 10 s in continuous mode (InGaAlP laser, Twin Laser®, MMOptics, Sao Carlos, Sao Paulo, Brazil). The teeth were extracted at the regular exfoliation period. The dentin-pulp complex was graded by an established histopathological score system. Statistical analysis was performed by Kruskal-Wallis and chi-square test. The histopathological assessment revealed statistically significant differences among groups (P < 0.05). The lowest degree of pulpal inflammation was present in LLLT + calcium hydroxide (P = 0.0296). Calcium hydroxide showed the highest rate of hard tissue barrier (P = 0.0033), odontoblastic layer (P = 0.0033), and dense collagen fibers (P = 0.0095). On the other hand, formocresol showed the highest incidence of internal resorption (P = 0.0142). Based on this study, low-level laser therapy preceding the use of calcium hydroxide exhibited satisfactory results on pulp tissue healing. However, further clinical studies on human teeth with long-term follow-up are needed to test the low-level laser therapy efficacy.

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Acknowledgments

The authors would like to thank all the volunteers and the financial support of the Sao Paulo Research Foundation (FAPESP grant nos. 2009/11284-4 to TMO). The authors would like to thank Daniele Santi Ceolin and Patrícia De Sá Mortágua Gemino for their excellent laboratorial assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry - University of São Paulo, Bauru, SP, Brazil

    Nádia Carolina Teixeira Marques, Natalino Lourenço Neto, Ana Paula Fernandes, Maria Aparecida Andrade Moreira Machado & Thais Marchini Oliveira

  2. Department of Biology Science, Bauru School of Dentistry - University of São Paulo, Bauru, SP, Brazil

    Camila de Oliveira Rodini

  3. Department of Clinics and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil

    Vivien Thiemy Sakai

Authors
  1. Nádia Carolina Teixeira Marques
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  2. Natalino Lourenço Neto
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  3. Camila de Oliveira Rodini
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  4. Ana Paula Fernandes
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  5. Vivien Thiemy Sakai
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  6. Maria Aparecida Andrade Moreira Machado
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  7. Thais Marchini Oliveira
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Correspondence to Thais Marchini Oliveira.

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Marques, N.C.T., Neto, N.L., Rodini, C.d.O. et al. Low-level laser therapy as an alternative for pulpotomy in human primary teeth. Lasers Med Sci 30, 1815–1822 (2015). https://doi.org/10.1007/s10103-014-1656-7

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  • DOI: https://doi.org/10.1007/s10103-014-1656-7

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