Abstract
The aim of this study is to analyze the effects of low-level laser therapy (LLLT) on the regeneration of the sciatic nerve in rats following a complete nerve resection. Male Wistar rats were divided into a control injury group, injury groups irradiated with a 660-nm laser at 10 or 50 J/cm2, and injury groups irradiated with an 808-nm laser at 10 or 50 J/cm2. Treatment began 24 h following nerve resection and continued for 15 days. Using the sciatic functional index (SFI), we show that the injured animals treated with 660 nm at 10 and 50 J/cm2 had better SFI values compared with the control injury and the 808-nm groups. Animals irradiated with the 808-nm laser at 50 J/cm2 show higher values for fiber density than do control animals. In addition, axon and fiber diameters were larger in animals irradiated with 660 nm at 50 J/cm2 compared to the control group. These findings indicate that 660-nm LLLT is able to provide functional gait recovery and leads to increases in fiber diameter following sciatic nerve resection.
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References
Ferrigno ISV, Freitas PP, Freitas AD (2005) Peripheral nerve injuries. In: Freitas PP (ed) Hand rehabilitation, 1st edn. Atheneu, São Paulo, pp 211–254
Gigo-Benato D, Russo TL, Tanaka EH, Assis L, Salvini TF, Parizotto NA (2010) Effects of 660 and 780-nm low-level laser therapy on neuromuscular recovery after crush injury in rat sciatic nerve. Lasers Surg Med 42:673–682
Belchior AC, dos Reis FA, Nicolau RA, Silva IS, Perreira DM, de Carvalho PT (2009) Influence of laser (660 nm) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci 24:893–899
Amado S, Rodrigues JM, Luís AL, Armada-da-Silva PA, Vieira M, Gartner A, Simões MJ, Veloso AP, Fornaro M, Raimondo S, Varejão AS, Geuna S, Maurício AC (2010) Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair. J Neuroeng Rehabil 11:7–7
Reis FA, Belchior ACG, Nicolau RA, Fonseca TS, Carvalho PTC (2008) Efeito da terapia com laser de arseneto de gálio e alumínio (660 nm) sobre a recuperação do nervo ciático de ratos após lesão por neurotmese seguida de anastomose epineural: análise funcional. Braz J Phys Ther 12:215–221
Wells MR, Kraus K, Batter DK, Blunt DG, Weremowitz J, Lynch SE, Antoniades HN, Hansson HA (1997) Gel matrix vehicles for growth factor application in nerve gap injuries repaired with tubes: a comparison of biomatrix, collagen, and methylcellulose. Exp Neurol 146:395–402
Izzo AD, Walsh JT Jr, Ralph H, Webb J, Bendett M, Wells J, Richter C-P (2008) Laser stimulation of auditory neurons: effect of shorter pulse duration and penetration depth. Biophys J 94:3159–3166
Rochkind S (2009) Phototherapy in peripheral nerve regeneration: from basic science to clinical study. Neurosurg Focus 26:E8
Barbosa RI, Marcolino AM, Guirro RJ, Mazzer N, Barbieri CH, Fonseca MR (2010) Comparative effects of wavelengths of low-power laser in regeneration of sciatic nerve in rats following crushing lesion. Lasers Med Sci 25:423–430
Rochkind S, Leider-Trejo L, Nissan M, Shamir MH, Kharenko O, Alon M (2007) Efficacy of 780-nm laser phototherapy on peripheral nerve regeneration after neurotube reconstruction procedure (double-blind randomized study). Photomed Laser Surg 25:137–143
Bagis S, Comelekoglu U, Coskun B, Milcan A, Buyukakilli B, Sahin G, Ozisik S, Erdogan C (2003) No effect of GA-AS (904 nm) laser irradiation on the intact skin of the injured rat sciatic nerve. Lasers Med Sci 18:83–88
Fernandes M, Valente SG, Fernandes MJ, Félix EP, Mazzacoratti Mda G, Scerni DA, dos Santos JB, Leite VM, Faloppa F (2008) Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion. J Neurosci Methods 170:16–24
Grzesiak-Janas G, Janas A (2001) Conservative closure of antro-oral communication stimulated with laser light. J Clin Laser Med Surg 19(4):181–184
De Medinaceli L, Freed WJ, Wyatt RJ (1982) An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol 77:634–643
Bain JR, Mackinnon SE, Hunter DA (1989) Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg 83:129–138
Madison RD, Robinson GA, Chadaram SR (2007) The specificity of motor neurone regeneration (preferential reinnervation). Acta Physiol (Oxf) 189:201–206
English AW (2005) Enhancing axon regeneration in peripheral nerves also increases functionally inappropriate reinnervation of targets. J Comp Neurol 490:427–441
Ehrlicher A, Betz T, Stuhrmann B, Koch D, Milner V, Raizen MG, Käs J (2002) Guiding neuronal growth with light. Proc Natl Acad Sci USA 99:16024–16028
Renno ACM, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275–280
Karu TI, Lubart R (2000) Effects of low-power light on biological systems V. Proceedings of SPIE, vol 41, Amsterdam, The Netherlands, 17 pp
Mognato M, Squizzato F, Facchin F, Zaghetto L, Corti L (2004) Cell growth modulation of human cells irradiated in vitro with low-level laser therapy. Photomed Laser Surg 22:523–526
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Medalha, C.C., Di Gangi, G.C., Barbosa, C.B. et al. Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats. Lasers Med Sci 27, 629–635 (2012). https://doi.org/10.1007/s10103-011-1008-9
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DOI: https://doi.org/10.1007/s10103-011-1008-9