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Two-layered multiphoton microscopic imaging of cervical tissue

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Abstract

We demonstrate the application of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals in imaging two-layered structures of cervical tissue in mice. It was found that MPM is effective for visualizing two-layered cervical microstructures, monitoring the metabolic activity and the density (nuclear-to-cytoplasmic) of epithelial cells, measuring the size of epithelial cell nuclei and the thickness of the epithelial layer, and quantifying collagen in the stroma. These results suggest that, with the advent of clinical portability of typical multiphoton endoscopes, the MPM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of cervical cancer.

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Acknowledgments

The project was supported by the National Natural Science Foundation of China (grant nos. 60508017,60678054, 60711120031), the Natural Science Foundation of the Fujian Province of China (grants 2007J0007, C0720001) and the Program for New Century Excellent Talents in University (NCET-07-0191).

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Authors and Affiliations

  1. Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University, Ministry of Education, Fuzhou, 350007, China

    Shuangmu Zhuo, Jianxin Chen, Tianshu Luo, Xingshan Jiang, Shusen Xie & Rong Chen

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  1. Shuangmu Zhuo
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  2. Jianxin Chen
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  3. Tianshu Luo
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  4. Xingshan Jiang
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  5. Shusen Xie
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  6. Rong Chen
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Correspondence to Jianxin Chen.

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Zhuo, S., Chen, J., Luo, T. et al. Two-layered multiphoton microscopic imaging of cervical tissue. Lasers Med Sci 24, 359–363 (2009). https://doi.org/10.1007/s10103-008-0570-2

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