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Laser phototherapy effect on protein metabolism parameters of rat salivary glands

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Abstract

The aim of this study was to evaluate the effects of infrared diode laser phototherapy (LP) on tissues of the submandibular gland (SMG) and parotid gland (PG). Wistar rats were randomly divided into experimental (A and B) and control (C) groups. A diode laser, 808 nm wavelength, in continuous wave mode, was applied to the PG, SMG and sublingual gland in the experimental groups on two consecutive days. The doses were 4 J/cm2 and 8 J/cm2, and total energy was 7 J and 14 J, respectively. The power output (500 mW) and power density (277 mW/cm2) were the same for both experimental groups. In order to visualize the area irradiated by the infrared laser, we used a red pilot beam (650 nm) with 3 mW maximum power for the experimental groups. For the control group, the red pilot beam was the only device used. The SMG and PG were removed after 1 week of the first irradiation. Total protein concentration, amylase, peroxidase, catalase and lactate dehydrogenase assays were performed, as well as histological analysis. Statistical tests revealed significant increase in the total protein concentration for groups A and B in the parotid glands (P < 0.05). Based on the results of this study, LP altered the total protein concentration in rats’ parotid glands.

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Acknowledgments

The authors wish to express their gratitude to ZAP Lasers (USA) for the laser equipment and to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support.

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Authors and Affiliations

  1. Oral Biology Research Center, Faculty of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo, SP, 05508–000, Brazil

    Alyne Simões & José Nicolau

  2. Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, USA

    Walter Luiz Siqueira

  3. Cell and Molecular Biology Group, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, Brazil

    Marcelo Lazzaron Lamers & Marinilce Fagundes Santos

  4. Special Laboratory of Laser in Dentistry, LELO, Faculty of Dentistry, University of São Paulo (USP), São Paulo, Brazil

    Carlos de Paula Eduardo

Authors
  1. Alyne Simões
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  2. Walter Luiz Siqueira
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  3. Marcelo Lazzaron Lamers
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  4. Marinilce Fagundes Santos
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  5. Carlos de Paula Eduardo
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  6. José Nicolau
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Correspondence to Alyne Simões.

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Simões, A., Siqueira, W.L., Lamers, M.L. et al. Laser phototherapy effect on protein metabolism parameters of rat salivary glands. Lasers Med Sci 24, 202–208 (2009). https://doi.org/10.1007/s10103-008-0548-0

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  • DOI: https://doi.org/10.1007/s10103-008-0548-0

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