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Sensitivity and specificity of a new commercial enzyme-linked immunoassay kit for detecting Entamoeba histolyticaIgG antibodies in serum samples

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Abstract

The study presented here was conducted to evaluate the performance of the newly available RIDASCREEN Set (R-Biopharm AG, Darmstadt, Germany) for the detection of immunoglobulin G antibodies against Entamoeba histolytica. The sensitivity and specificity of this new enzyme-linked immunosorbent assay were evaluated using a panel of sera from 239 individuals. The assay was positive for 43 of 44 patients with invasive amebiasis, including all 18 patients with amebic liver abscess, while it was negative for 190 of 195 adult controls who were either healthy individuals or patients with other parasitic diseases. The kit was found to be highly specific (97.4%) and sensitive (97.7%) for detecting antibodies against E. histolytica in humans. Although antibody titers in patients with amebic liver abscess tend to be higher on average than in patients with invasive amebiasis, it is not possible to distinguish the two forms solely based on the results of this commercial test.

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  1. Berlin Institute of Tropical Medicine, Spandauer Damm 130, 14050, Berlin, Germany

    M. Knappik, U. Börner & T. Jelinek

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  1. M. Knappik
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  2. U. Börner
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  3. T. Jelinek
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Correspondence to T. Jelinek.

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Knappik, M., Börner, U. & Jelinek, T. Sensitivity and specificity of a new commercial enzyme-linked immunoassay kit for detecting Entamoeba histolyticaIgG antibodies in serum samples. Eur J Clin Microbiol Infect Dis 24, 701–703 (2005). https://doi.org/10.1007/s10096-005-0030-x

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  • DOI: https://doi.org/10.1007/s10096-005-0030-x

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