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The effects of IVIg therapy on serum levels of neuropeptide Y and cytokines in Guillain-Barré syndrome

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Abstract

Background

Guillain-Barré syndrome (GBS) is a common acute immune-mediated inflammatory disorder affecting the peripheral nervous system (PNS) of humans. Studies in humans and in animal models revealed that neuropeptide Y (NPY) levels are altered in some neurodegenerative and neuroimmune disorders. Herein, we investigated the levels of NPY and cytokines in the serum of GBS patients and explored the roles of NPY in the disease severity and its short-term prognosis.

Methods

Twenty patients with GBS (case group) and twenty healthy individuals (control group) were enrolled in this study. NPY levels were analyzed by enzyme-linked immunosorbent assay (ELISA). The levels of pro- and anti-inflammatory cytokines (including interferon-γ (IFN-γ), interleukin (IL)-4, IL-10, IL-12p70, IL-17A, and tumor necrosis factor-α (TNF-α)) were analyzed using cytometric beads array (CBA). The clinical characteristics, disease severity, and short-term prognosis were compared between the two groups.

Results

Compared with the control group, the levels of NPY and cytokines were significantly increased in the serum of patients with GBS. NPY levels in the serum of GBS patients were correlated with the disease severity.

Conclusion

Our results suggest that NPY and cytokines are involved in the pathogenesis of GBS. The levels of NPY can help to predict the severity of the disease.

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Abbreviations

GBS:

Guillain-Barré syndrome

PNS:

Peripheral nervous system

NPY:

Neuropeptide Y

ELISA:

Enzyme-linked immunosorbent assay

IFN-γ:

Interferon-γ

IL:

Interleukin

TNF-α:

Tumor necrosis factor-α

CBA:

Cytometric beads array

CNS:

Central nervous system

MS:

Multiple sclerosis

IVIg:

Intravenous immunoglobulin

HFGS:

Hughes Functional Grading Scale

MRC:

Medical Research Council

SPSS:

Statistical Product and Service Solutions

EAN:

Experimental autoimmune neuritis

PE:

Phycoerythrin

Th:

Helper T cell

TGF-β:

Transforming growth factor β

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Funding

This study was supported by grants from The First Hospital, Jilin University Changchun, China General Program of the National Natural Science Foundation (No. 81771299).

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Authors and Affiliations

  1. Neuroscience Center, Department of Neurology, The First Hospital of Jilin University, Jilin University, Xinmin Street 71#, Changchun, 130021, China

    Chunrong Li, Tianfei Luo, Yanwei Cheng, Shan Liu, Lifan Qiao, Xiujuan Wu & Kangding Liu

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  1. Chunrong Li
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  2. Tianfei Luo
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  3. Yanwei Cheng
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  4. Shan Liu
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  5. Lifan Qiao
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  6. Xiujuan Wu
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  7. Kangding Liu
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Corresponding author

Correspondence to Kangding Liu.

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This study was approved by the ethics committee of the First Hospital of Jilin University, Changchun, China.

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The authors declare that they have no conflicts of interest.

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Informed consent was obtained from all individual participants included in the study.

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Li, C., Luo, T., Cheng, Y. et al. The effects of IVIg therapy on serum levels of neuropeptide Y and cytokines in Guillain-Barré syndrome. Neurol Sci 41, 295–303 (2020). https://doi.org/10.1007/s10072-019-04063-3

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  • DOI: https://doi.org/10.1007/s10072-019-04063-3

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