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Whole-cell bioconversion using non-Leloir transglycosylation reactions: a review

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Abstract

Microbial biocatalysts are evolving technological tools for glycosylation research in food, feed and pharmaceuticals. Advances in bioengineered Leloir and non-Leloir carbohydrate-active enzymes allow for whole-cell biocatalysts to curtail production costs of purified enzymes while enhancing glucan synthesis through continued enzyme expression. Unlike sugar nucleotide-dependent Leloir glycosyltransferases, non-Leloir enzymes require inexpensive sugar donors and can be designed to match the high value, yield and selectivity of the former. This review addresses the current state of bacterial cell-based production of glucans and glycoconjugates via transglycosylation, and describes how alterations made to microbial hosts to surpass purified enzymes as the preferred mode of catalysis are steadily being acquired through genetic engineering, rational design and process optimization. A comprehensive exploration of relevant literature has been summarized to describe whole-cell biocatalysis in non-Leloir glycosylation reactions with various donors and acceptors, and the characterization, application and latest developments in the optimization of their use.

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Funding

This work was supported by the National Research Foundation of Korea (NRF). Additionally, it was funded by the Korean government (MSIT, Ministry of Science and ICT) (Grant Nos. 2021R1C1C1004489 and 2021R1A4A1023437).

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Authors and Affiliations

  1. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Inonge Noni Siziya & Dong-Ho Seo

  2. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Inonge Noni Siziya & Myung-Ji Seo

  3. Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Jong-Hyun Jung

  4. Research Group of Consumer Safety, Korea Food Research Institute (KFRI), Jeollabuk-do, 55365, Korea

    Min-Cheol Lim

  5. Department of Food Science and Biotechnology, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Dong-Ho Seo

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Siziya, I.N., Jung, JH., Seo, MJ. et al. Whole-cell bioconversion using non-Leloir transglycosylation reactions: a review. Food Sci Biotechnol 32, 749–768 (2023). https://doi.org/10.1007/s10068-023-01283-4

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  • DOI: https://doi.org/10.1007/s10068-023-01283-4

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