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Modulation of gut microbiota by rice starch enzymatically modified using amylosucrase from Deinococcus geothermalis

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Abstract

Amylosucrase can increase the amount of resistant starch (RS) in starch by transferring glucose from sucrose to amylopectin. Here, rice starch was modified using amylosucrase from Deinococcus geothermalis (DgAS). DgAS-modified rice starch (DMRS) increased the side-chain length of amylopectin and appeared in the form of B-type crystals. In vitro digestion analyses revealed that DMRS had a higher RS contents and lower digestion rate than native rice starch. When high-fat diet (HFD)-induced C57BL/6 mice were orally administered DMRS, body weight and white fat tissues of DMRS-fed HFD mice were not significantly different. However, serum leptin and glucose levels were significantly decreased and serum glucagon like peptide-1was increased in these mice. The cecal microbiome in DMRS-fed HFD mice was identified to investigate the role of DMRS in gut microbiota regulation. DMRS supplementation increased the relative abundance of Bacteroides, Faecalibaculum, and Ruminococcus in mouse gut microbiota.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grants (Grant Nos. 2021R1C1C1004489 and 2021R1A4A1023437) and the Main Research Program (Grant No. E0170600‐06) of the Korea Food Research Institute (KFRI) funded by the Korean government (MSIT, Ministry of Science and ICT)

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  1. Eun-Ji Song and Eun-Sook Lee have contributed equally to this work.

Authors and Affiliations

  1. Research Group of Personalized Diet, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    Eun-Ji Song & Young-Do Nam

  2. Department of Pharmacology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea

    Eun-Sook Lee

  3. Department of Food Science and Biotechnology, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Dong-Ho Seo

  4. Department of Food Science and Technology, College of Agriculture and Life Sciences Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Yun-Sang So, Chang-Young Lee & Dong-Ho Seo

  5. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 13120, Republic of Korea

    Byung-Hoo Lee

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Song, EJ., Lee, ES., So, YS. et al. Modulation of gut microbiota by rice starch enzymatically modified using amylosucrase from Deinococcus geothermalis. Food Sci Biotechnol 32, 565–575 (2023). https://doi.org/10.1007/s10068-022-01238-1

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