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Synthesis of hexyl butyrate (apple and citrus aroma) by Candida rugosa lipase immobilized on Diaion HP-20 using the Box-Behnken design

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Abstract

This study aims at the synthesis of hexyl butyrate by Candida rugosa lipase (CRL) immobilized on Diaion HP 20. The lipase load used was 28.7 ± 2.1 mg/g (mg of lipase/g of support), whose hydrolytic activity was 132.0 ± 2.5 U/g. To obtain the maximum production of hexyl butyrate, the Box-Behnken design statistical planning was used, having as independent variables; biocatalyst concentration, temperature and acid:alcohol molar ratio and ester conversion as a dependent variable at 60, 180 and 480 min. For 60 min, 90.8% conversion was obtained at 47.25 ºC, 1:1.4 molar ratio and 17.65% of biocatalyst; 180 min, 94.5% conversion at 59.5 ºC, 1:2 molar ratio and 15.8% biocatalyst; 480 min, 95.01% conversion at 47.0 ºC, 1:2 molar ratio and 16.9% biocatalyst. CRL-Diaion HP 20 retained 60% of its initial activity after ten cycles of reactions showing potential for industrial use. The ester produced was identified by gas chromatography analyses.

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Acknowledgements

The authors would like to thank Coordination for the Improvement of Higher Education Personnel (CAPES) for their financial support and the State University of Santa Cruz (UESC) for its administrative and technical support.

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Authors and Affiliations

  1. Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga, 45700-000, Brazil

    Marta Maria Oliveira dos Santos, Luiz Henrique Sales de Menezes, Eliézer Luz do Espirito Santo, Márcia Soares Gonçalves & Iasnaia Maria de Carvalho Tavares

  2. Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, 45654-370, Brazil

    Marise Silva de Carvalho, Luiz Carlos Salay, Marcelo Franco & Julieta Rangel de Oliveira

  3. Institute of Chemistry, Federal University of Alfenas, Alfenas, 37130-001, Brazil

    Adriano Aguiar Mendes

  4. Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, COAH, Mexico

    Héctor A. Ruiz

Authors
  1. Marta Maria Oliveira dos Santos
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  2. Luiz Henrique Sales de Menezes
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  3. Eliézer Luz do Espirito Santo
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  4. Marise Silva de Carvalho
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  7. Adriano Aguiar Mendes
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  8. Héctor A. Ruiz
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  9. Luiz Carlos Salay
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Correspondence to Julieta Rangel de Oliveira.

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dos Santos, M.M.O., de Menezes, L.H.S., do Espirito Santo, E.L. et al. Synthesis of hexyl butyrate (apple and citrus aroma) by Candida rugosa lipase immobilized on Diaion HP-20 using the Box-Behnken design. Food Sci Biotechnol 32, 689–696 (2023). https://doi.org/10.1007/s10068-022-01200-1

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