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Repressive effects of red bean, Phaseolus angularis, extracts on obesity of mouse induced with high-fat diet via downregulation of adipocyte differentiation and modulating lipid metabolism

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Abstract

Obesity is generally caused by quantitative changes in adipocyte differentiation and fat metabolism. Only a few studies have been determined the effect of red beans extract on obesity and plasma cholesterol concentration. We have been studied the functional activities of red-bean extracts including anti-oxidative effect against DNA and cell damages. Histological study including micro CT analysis showed that the accumulation of fat in hepatocytes and intestines was significantly decreased in red bean extract treated group. In addition, plasma cholesterol and triglyceride levels were decreased in blood samples. In addition, it was confirmed that the red bean extract inhibited the expression of PPARγ, Fabp4 and RETN genes, which regulate total adipocyte differentiation and lipid metabolism. Red bean extract inhibits the expressions of transcription factors associated with adipocyte differentiation in a dose-dependent manner, thereby inhibiting fat accumulation and decreasing blood lipid levels in obese mice induced by high fat diet.

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Abbreviations

FAA:

Formaldehyde–acetic acid–ethanol

TMB:

Tetramethylbenzidine

APOE:

Apolipoprotein E

Fabp4:

Fatty acid binding protein 4

Scd1:

Adipsin and stearoyl-coenzymeA desaturase 1

PPARγ:

Peroxisome proliferator-activated receptor gamma

RETN:

Resistin

AST:

Aspartate aminotransferase

ALT:

Alanine aminotransferase

IBMX:

3-Isobutyl-1-methylxanthine

DEPC:

Diethyl pyrocarbonate

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Acknowledgements

This work supported by a Grant from 2016 Research Funds of Andong National University.

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Authors and Affiliations

  1. Department of Biological Science, Andong National University, 1375 Gyungdong-ro, Andong, 36728, South Korea

    Young Mi Park, Jae-Hwan Lim, Jong Eun Lee & Eul-Won Seo

  2. Institute of Marine Biotechnology, Andong National University, Andong, 36728, South Korea

    Jee In Kim & Eul-Won Seo

  3. Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, 41566, South Korea

    Jee In Kim & Je-Yong Choi

  4. Biotechnology R&D Center, Cell Biotech, Gimpo, 10003, South Korea

    Dong Hyun Seo

  5. Department of Korean Neuropsychiatry, National Medical Center, Seoul, 04564, South Korea

    Joo Hee Seo

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  1. Young Mi Park
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  2. Jee In Kim
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  3. Dong Hyun Seo
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Correspondence to Eul-Won Seo.

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This study protocol was reviewed and approved by the institutional review board of the Andong National University (2014-3-1111-09-01).

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None of the authors of this study has any financial interest or conflict with industries or parties.

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Park, Y.M., Kim, J.I., Seo, D.H. et al. Repressive effects of red bean, Phaseolus angularis, extracts on obesity of mouse induced with high-fat diet via downregulation of adipocyte differentiation and modulating lipid metabolism. Food Sci Biotechnol 27, 1811–1821 (2018). https://doi.org/10.1007/s10068-018-0421-2

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