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Immuno-enhancement effect of polysaccharide extracted from Stichopus japonicus on cyclophosphamide-induced immunosuppression mice

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Abstract

Polysaccharide (SJP) was extracted from Sea cucumber, Stichopus japonicas, and its immune-enhancing activities were evaluated in vivo immune-suppressed mice systems. Cyclophosphamide(CY)-treated mice were orally administrated with SJP according to different concentrations. The results showed that administration of SJP significantly increased spleen index without variation of the body weight, compared to only CY treatment group. The proliferation of splenic lymphocyte and NK activity was also stimulated by SJP. In addition, the oral administration of SJP up-regulated COX-2 and TLR-4 as well as cytokines such as IL-1β, IL-4, IL-6, IL-10, TNF-α and IFN-γ, which are secreted from splenic lymphocytes in cyclophosphamide-treated mice. Moreover, our results showed that SJP stimulated macrophages via NF-κB and MAPK signaling pathways. These findings provided the potential use of SJP as an alternative means under immune-suppressed conditions, and furthermore can be utilized as a functional material for food and pharmaceutical industries.

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Acknowledgements

This study was supported by the Marine Bio-Regional Specialization Leading Technology Development Program (D11413914H480000100) funded by the Ministry of Oceans and Fisheries in Korea.

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Authors and Affiliations

  1. Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Korea

    Chaiwat Monmai, Sung Hee Park, SangGuan You & Woo Jung Park

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  1. Chaiwat Monmai
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  2. Sung Hee Park
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  4. Woo Jung Park
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Correspondence to Woo Jung Park.

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Monmai, C., Park, S.H., You, S. et al. Immuno-enhancement effect of polysaccharide extracted from Stichopus japonicus on cyclophosphamide-induced immunosuppression mice. Food Sci Biotechnol 27, 565–573 (2018). https://doi.org/10.1007/s10068-017-0248-2

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  • DOI: https://doi.org/10.1007/s10068-017-0248-2

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