Skip to main content
SpringerLink
Log in

Beneficial effect of persimmon leaves and bioactive compounds on thrombosis

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The effects of ethanol extracts of persimmon leaves (EPL) and the major flavonoids catechin, epicatechin, and epicatechin gallate on blood coagulation and platelet aggregation were evaluated. Anticoagulant activities were examined by monitoring the activated partial thromboplastin time (aPTT) and the prothrombin time (PT). EPL significantly prolonged PT and aPTT. The aPTT was significantly increased in the presence of catechin, epicatechin, and epicatechin gallate, compared with controls. In order to determine the antiplatelet activity, arachidonic acid (AA), collagen, and adenosine diphosphate (ADP)-induced platelet aggregation were examined. PL and the major flavonoids significantly reduced AA-induced platelet aggregation, although they did not significantly affect ADP and collagen-induced platelet aggregation, compared with controls. EPL and the major flavonoids significantly inhibited serotonin, thromboxane A2, and soluble P-selectin generation, compared with controls. PL has potential for prevention and improvement of thrombosis by inhibiting blood coagulation and platelet activation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Furie B, Furie BC. Mechanisms of thrombus formation. New Engl. J. Med. 359: 938–949 (2008)

    Article  CAS  Google Scholar 

  2. Furie B, Furie BC. Thrombus formation in vivo. J. Clin. Invest. 115: 3355–3362 (2005)

    Article  CAS  Google Scholar 

  3. Gopinath SCB, Shikamoto Y, Mizuno H, Kumar PK. A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway. Thromb. Haemostasis 95: 767–771 (2006)

    CAS  Google Scholar 

  4. Gopinath SCB, Shikamoto Y, Mizuno H, Kumar PK. Snake-venom-derived Factor IX-binding protein specifically blocks the gamma-carboxyglutamic acid-rich-domain-mediated membrane binding of human Factors IX and X. Biochem. J. 405: 351–357 (2007)

    Article  CAS  Google Scholar 

  5. Charles TE. The interactions between inflammation and coagulation. Brit. J. Haematol. 131: 417–430 (2005)

    Article  Google Scholar 

  6. Kim SY, Koo YK, Koo JY, Ngoc TM, Kang SS, Bae K, Kim YS, Choi HSY. Platelet anti-aggregation activities of compound from Cinnamomum cassia. J. Med. Food 13: 1069–1074 (2010)

    Article  CAS  Google Scholar 

  7. Yu HY, Park SW, Chung IM, Jung YS. Anti-platelet effects of yuzu extract and its component. Food Chem. Toxicol. 49: 3018–3024 (2011)

    Article  CAS  Google Scholar 

  8. Jennings LK. Mechanisms of platelet activation: Need for new strategies to protect against platelet-mediated atherothrombosis. Thromb. Haemostasis 102: 248–257 (2009)

    CAS  Google Scholar 

  9. Cho HJ, Kittaka R, Abdou AM, Kim M, Kim HS, Lee DH, Park HJ. Inhibitory effects of oligopeptides from hen egg white on both human platelet aggregation and blood coagulation. Arch. Pharm. Res. 32: 945–953 (2009)

    Article  CAS  Google Scholar 

  10. Konkle BA. Bleeding and thrombosis. 17th ed. pp. 369–369. In: Harrison’s Principles of Internal Medicine. Fauci AS, Braunwald E, Kasper DL. Hauser SL, Longo DL, Jameson JL, Loscalzo J (eds). McGraw Hill, New York, NY, USA (2008)

    Google Scholar 

  11. Badimon JJ, Weng D, Chesebro JH, Fuster V, Badimon L. Platelet deposition induced by severely damaged vessel wall is inhibited by a boroarginine synthetic peptide with antithrombin activity. Thromb. Haemostasis 71: 511–516 (1994)

    CAS  Google Scholar 

  12. Kang WS, Lim IH, Yuk DY, Chung KH, Park JB, Yoo HS, Yun YP. Antithrombotic activities of green tea catechins and (−)-epigallocatechin gallate. Thromb. Res. 96: 229–237 (1999)

    Article  CAS  Google Scholar 

  13. Burger PC, Wagner DD. Platelet P-selectin facilitates atherosclerotic lesion development. Blood 101: 2661–2666 (2003)

    Article  CAS  Google Scholar 

  14. Fan J, Zhang Y, Chang X, Zhang B, Jiang D, Saito M, Li Z. Antithrombotic and fibrinolytic acitivities of methanolic extract of aged sorghum vinegar. J. Agr. Food Chem. 57: 8683–8687 (2009)

    Article  CAS  Google Scholar 

  15. Gopinath SCB. Anti-coagulant aptamers. Thromb. Res. 122: 838–847 (2008)

    Article  CAS  Google Scholar 

  16. Tanaka KA, Key NS, Levy JH. Blood coagulation: Hemostasis and thrombin regulation. Anesth. Analg. 108: 1433–1446 (2009)

    Article  CAS  Google Scholar 

  17. Hu FB. Plant-based foods and prevention of cardiovascular disease: An overview. Am. J. Clin. Nutr. 78: 544S–551S (2003)

    CAS  Google Scholar 

  18. Kim KY, Lim KM, Kim CW, Shin HY, Seo DB, Lee SJ, Noh JY, Bae ON, Sin S, Chung JH. Black soybean extract can attenuate thrombosis through inhibition of collagen-induced platelet activation. J. Nutr. Biochem. 22: 964–970 (2011)

    Article  CAS  Google Scholar 

  19. Jung UJ, Park YB, Kim SR, Choi MS. Supplementation of persimmon leaf ameliorates hyperglycemia, dyslipidemia and hepatic fat accumulation in type 2 diabetic mice. PloS One 11: e49030 (2012)

    Article  Google Scholar 

  20. Morel I, Lescoat G, Cogrel P, Sergent O, Pasdeloup N, Brissot P, Cillard P, Cillard J. Antioxidant and iron-chelating activities of the flavonoids catechin, quercetin and diosmetin on iron-loaded rat hepatocyte cultures. Biochem. Pharmacol. 45: 13–19 (1993)

    Article  CAS  Google Scholar 

  21. Sa YS, Kim SJ, Choi HS. The anticoagulant fraction from the leaves of Diospyros kaki L. has an antithrombotic activity. Arch. Pharm. Res. 28: 667–674 (2005)

    Article  CAS  Google Scholar 

  22. Miao M, Zhang X, Wang L. Persimmon leaf flavonoid induces brain ischemic tolerance in mice. Neural. Regen. Res. 8: 1376–1382 (2013)

    CAS  Google Scholar 

  23. Dejana E, Quintana A, Callioni A, de Gaetano G. Bleeding time in laboratory animals. III-Do tail bleeding times in rats only measure a platelet defect? (the aspirin puzzle). Thromb. Res. 15: 199–207 (1979)

    Article  CAS  Google Scholar 

  24. Born GVR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194: 927–929 (1962)

    Article  CAS  Google Scholar 

  25. World Health Organization. The top 10 causes of death. Available from: http://www.who.int/mediacentre/factsheets/fs310/en/. Accessed May 31, 2013.

    Google Scholar 

  26. Barrett NE, Holbrook L, Jones S, Kaiser WJ, Moraes LA, Rana R, Sage T, Stanley RG, Tucker KL, Wright B, Gibbins JM. Future innovations in anti-platelet therapies. Brit. J. Pharmacol. 154: 918–939 (2009)

    Article  Google Scholar 

  27. Lee SM, Moon J, Chung JH, Cha YJ, Shin MJ. Effect of quercetin-rich onion peel extracts on arterial thrombosis in rats. Food. Chem. Toxicol. 57: 99–105 (2013)

    Article  CAS  Google Scholar 

  28. Heemskerk JWM, Bevers EM, Lindhout T. Platelet acitivation and blood coagulation. Thromb. Haemostasis 88: 196–193 (2002)

    Google Scholar 

  29. Gratacap MP, Hérault JP, Viala C, Ragab A, Savi P, Herbert JM, Chap H, Plantavid M, Payrastre B. FcγRIIA requires a Gi-dependent pathway for an efficient stimulation of phosphoinositide 3-kinase, calcium mobilization, and platelet aggregation. Blood 96: 3439–3446 (2000)

    CAS  Google Scholar 

  30. Lee W, Yang EJ, Ku SK, Song KS, Bae JS. Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions. BMB Rep. 45: 390–395 (2012)

    Article  CAS  Google Scholar 

  31. Whiteheart SW. Platelet granules: Surprise packages. Blood 118: 1190–1191 (2011)

    Article  CAS  Google Scholar 

  32. Clutton P, Folts JD, Freedman JE. Pharmacological control of platelet function. Pharmacol. Res. 44: 255–264 (2001)

    Article  CAS  Google Scholar 

  33. Halushka PV, Allan CJ, Davis-Bruno KL. Thromboxane A2 receptors. J. Lipid Mediat. Cell 12: 361–378 (1995)

    Article  CAS  Google Scholar 

  34. Son DJ, Cho MR, Jin YR, Kim SY, Park YH, Lee SH, Akiba S, Sato T, Yun YP. Antiplatelet effect of green tea catechins: A possible mechanism through arachidonic acid pathway. Prostag. Leukotr. Ess. 71: 25–31 (2004)

    Article  CAS  Google Scholar 

  35. Jin YR, Im JH, Park ES, Cho MR, Han XH, Lee JJ, Lim Y, Kim TJ, Yun YP. Antiplatelet activity of epigallocatechin gallate is mediated by the inhibition of PLCgamma2 phosphorylation, elevation of PGD2 production, and maintaining calcium-ATPase activity. J. Cardiovasc. Pharm. 51: 45–54 (2008)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science and Nutrition, Kyungpook National University, Daegu, 702-701, Korea

    Ri Ryu, Yu-Ri Seo & Myung-Sook Choi

  2. Center for Food and Nutritional Genomics Research, Kyungpook National University, Daegu, 702-701, Korea

    Un Ju Jung & Myung-Sook Choi

  3. Foods R&D, CJ Cheil Jedang Corporation, Seoul, 100-400, Korea

    Hye-Jin Kim & Byoung Seok Moon

  4. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 702-701, Korea

    Jong-Sup Bae

  5. School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, Korea

    Dong Gun Lee

Authors
  1. Ri Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Un Ju Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu-Ri Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hye-Jin Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Byoung Seok Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jong-Sup Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dong Gun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Myung-Sook Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Myung-Sook Choi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ryu, R., Jung, U.J., Seo, YR. et al. Beneficial effect of persimmon leaves and bioactive compounds on thrombosis. Food Sci Biotechnol 24, 233–240 (2015). https://doi.org/10.1007/s10068-015-0031-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0031-1

Keywords

Search

Navigation