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Effect of extract from laquer tree (Rhus verniciflua Stokes) on DNCB-induced atopic dermatitis in NC/Nga mouse

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Abstract

Laquer tree (Rhus verniciflua Stokes; Anacardiaceae, RVS) has been used in oriental medicines. This study examined whether the extract of RVS free of urushiol (detoxified RVS extract, DRE) exerting antiinflammation could alleviate the symptoms of atopic dermatitis (AD) induced with 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. DRE contained flavonoids like fustin (153 mg/g), fisetin (14.6 mg/g), sulfuretin (13.3 mg/g), and quercetin (0.95 mg/g). DRE (10 μg/mL) decreased the production of nitric oxide activated by lipopolysaccharide. Oral administration of DRE (200 mg/kg weight/day) decreased scratching frequencies, the epidermal thickness on dorsal skin, and the number of degranulated mast cell. DRE significantly lowered the levels of IgE in sera (p<0.01), which were elevated by DNCB. DNCB reciprocally raised IL-4 and lowered IFN-γ in media of spleenocyte. DRE reversely raised IFN-γ and lowered IL-4. Contrastively, dexamethasone suppressed the overall immune reactions. In conclusion, DRE inhibited Th2-mediated IgE overproduction through modulation of Th1/2 balance, which was in part attributed to anti-inflammatory activity.

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Authors and Affiliations

  1. Graduate School of Biotechnology, Kyung Hee University, Yongin, Gyeonggi, 446-701, Korea

    Jung-Eun Yang, Jung Min Lee, Heon-Sub Shin, Sang-Yong Park, Don-Gil Lee & Tae-Hoo Yi

  2. Dr. Hoo Lifescience Inc., Kyung Hee University, 501 College of Life Science, Yongin, Gyeonggi, 446-701, Korea

    Gafurjon T. Mavlonov

  3. Department of Medical Engineering, College of Health Science, Eulji University, Seongnam, Gyeonggi, 461-713, Korea

    Woo-Cheol Lee

  4. Faculty of Biotechnology, Jeju National University, Jeju, Jeju, 690-756, Korea

    Somi Kim Cho

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  1. Jung-Eun Yang
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Correspondence to Tae-Hoo Yi.

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Yang, JE., Lee, J.M., Shin, HS. et al. Effect of extract from laquer tree (Rhus verniciflua Stokes) on DNCB-induced atopic dermatitis in NC/Nga mouse. Food Sci Biotechnol 21, 1321–1327 (2012). https://doi.org/10.1007/s10068-012-0174-2

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  • DOI: https://doi.org/10.1007/s10068-012-0174-2

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