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Purification of S-Alk(en)yl alka/enethiosulfinates of garlic (Allium sativum L.) by using recycling preparative HPLC

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Abstract

S-Alk(en)yl alka/enethiosulfinates formed in crushed garlic were purified by using recycling preparative HPLC. Allyl 2-propenethiosulfinate and methyl methanethiosulfinate were effective separated because no other thiosulfinates were coeluted with them. Allyl methanethiosulfinate and methyl 2-propenethiosulfinate, trans-1-propenyl methanethiosulfinate and methyl trans-1-propenethiosulfinate, (cis)- and (trans)-1-propenyl 2-propenethiosulfinate were eluted as 1 peak, respectively, and further separated by using additional kinds of column. Since the quantity of trans-1-propenyl trans-1-propenethiosulfinate (PPTHS) was small in garlic, PPTHS was isolated from a mixture of blanched onion homogenate and garlic alliinase and purified. Purified thiosulfinates can be employed for the study of antimicrobial activity, flavor, and greening of garlic.

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Correspondence to Kyu Hang Kyung.

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Lee, J.Y., Kyung, K.H. Purification of S-Alk(en)yl alka/enethiosulfinates of garlic (Allium sativum L.) by using recycling preparative HPLC. Food Sci Biotechnol 20, 1167–1170 (2011). https://doi.org/10.1007/s10068-011-0160-0

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  • DOI: https://doi.org/10.1007/s10068-011-0160-0

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