Abstract
Broussonetia papyrifera radix, fruits, leaves, and stems exhibit antioxidant, antinociceptive, antityrosinase, anti-inflammatory, and antiplatelet activities. However, study of the antioxidant activity of stem bark has been limited. In this work the antioxidant activity of stem bark and wood extracts were compared using different methods, and their phenolic constituents were analyzed. The bark ethyl acetate fraction exhibited the highest 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical, hydroxyl radical scavenging activity, and the highest total phenolic and flavonoid contents. Both bark and wood n-butanol fractions showed stronger superoxide anion radical scavenging activity and reducing potential than other fractions. The bark and wood hexane fraction provided the highest level of ion chelating power. The high performance liquid chromatography (HPLC) analysis reveals there are more phenolic compounds in bark than in wood. These findings suggest that bark exhibits a higher antioxidant capacity than wood and that ethyl acetate and n-butanol fractions of B. papyrifera bark are potential natural resources for pharmacology of functional foods.
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Biglari F, AlKarkhi AM, Easa AM. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chem. 107: 1636–1641 (2008)
Jiangsu New Medical College. Dictionary of Chinese Materia Medica. Vol. 1 [M]. Shanghai People’s Publishing House, Shanghai, China. pp. 2288–2290 (1977)
Kim SY, Kim JH, Kim SK, Oh MJ, Jung MY. Antioxidant activities of selected oriental herb extracts. J. Am. Oil Chem. Soc. 71: 633–640 (1994)
Matsuda H, Cai H, Kubo M, Tosa H, Iinuma M. Effects of Buddlejae Flos on in vitro aldose reductase activity. Biol. Pharm. Bull. 18: 463–466 (1995)
Kim YS, Park KH. Effects of ginseng saponins on morphine 6-dehydrogenase. Korean J. Pharmacogn. 25: 388–394 (1994)
Fang SC, Shieh BJ, Lin CN. Phenolic constituents of Formosan Broussonetia papyrifera. Phytochemistry 37: 851–853 (1994)
Fang SC, Shieh BJ, Wu RR, Lin CN. Isoprenylated flavonols of Formosan Broussonetia papyrifera. Phytochemistry 38: 535–537 (1995)
Fukai T, Ikuta J, Nomura T. Constituents of the cultivated mulberry trees of Broussonetia papyrifera. Chem. Pharm. Bull. 34: 1987–1993 (1986)
Zheng YH, Wang SY, Wang CY, Zheng W. Changes in strawberry phenolics, anthocyanins, and antioxidant capacity in response to high oxygen treatments. LWT-Food Sci. Technol. 40: 49–57 (2007)
Ragazzi E, Veronese G. Quantitative analysis of phenolic compounds after thin layer chromatographic separation. J. Chromatogr. 77: 369–375 (1973)
Park YK, Koo MH, Ikegaki M, Contado JL. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Arq. Biol. Technol. 40: 97–106 (1997)
Fernandes E, Borges F, Milhazes N, Carvalho FD, Bastos ML. Evaluation of superoxide radical scavenging activity of gallic acid and its alkyl esters using an enzymatic and a non-enzymatic system. Toxicol. Lett. 109(Supp.1): 42 (1999)
Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Bioph. Res. Co. 46: 849–854 (1972)
Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161–169 (1994)
Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem. 85: 611–616 (2004)
Soares JR, Dins TCP, Cunha AP, Ameida LM. Antioxidant activity of some extracts of Thymus zygis. Free Radical Res. 26: 469–478 (1997)
Brand W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm.-Wiss. Technol. 28: 25–30 (1995)
Mei RQ, Wang YU, Du GH, Liu GM, Zhang L, Cheng YX. Antioxidant lignans from the fruits of Broussonetia papyrifera. J. Nat. Prod. 72: 621–625 (2009)
Chun OK, Kim DO, Lee CY. Superoxide radical scavenging activity of the major polyphenols in fresh plums. J. Agr. Food Chem. 51: 8067–8072 (2003)
Oliveira I, Valentim C, Raquel B, Jose AP, Paula B. Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food Chem. Toxicol. 47: 1507–1511 (2009)
Dastmalchi HJ, Dorman D, Into L, Raimo H. Chemical composition and antioxidative activity of Moldavian balm (Dracocephalum moldavica L.) extracts. Lebensm.-Wiss. Technol. 40: 1655–1663 (2007)
Rice CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933–956 (1996)
Wang T, Rósa J, Guerún Ó. Total phenolic compounds, radical scavenging, and metal chelation of extracts from Icelandic seaweeds. Food Chem. 116: 240–248 (2009)
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Xu, ML., Wang, L., Hu, JH. et al. Antioxidant activities and related polyphenolic constituents of the methanol extract fractions from Broussonetia papyrifera stem bark and wood. Food Sci Biotechnol 19, 677–682 (2010). https://doi.org/10.1007/s10068-010-0095-x
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DOI: https://doi.org/10.1007/s10068-010-0095-x