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Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation

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Abstract

Artemisia lavandulaefolia has been utilized in traditional medicines for the treatment of several diseases. In this study, we attempted to determine whether the essential oil and 1,8-cineole of A. lavandulaefolia induce apoptosis in KB cells. The oil and 1,8-cineole induced the cell death of KB cells as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. The treatment of the cells with the oil also induced changes in the mitochondrial level of Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Furthermore, the oil increased the phosphorylation of extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase, and p38 MAPK, but 1,8-cineole activated pp38 and pJNK at the same concentrations. These findings show that the mitochondrial and MAPKs pathways might be involved in the oil-induced apoptosis, and may enhance our current understanding of the anticancer functions of the oil to a greater degree than 1,8-cineole.

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Authors and Affiliations

  1. Department of Dental Hygiene, Ulsan College, Ulsan, 680-749, Korea

    Youn-Hwa Kim & Ji-Young Kim

  2. Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, 120-749, Korea

    Jeong-Dan Cha

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  1. Jeong-Dan Cha
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  2. Youn-Hwa Kim
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Correspondence to Ji-Young Kim.

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Cha, JD., Kim, YH. & Kim, JY. Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation. Food Sci Biotechnol 19, 185–191 (2010). https://doi.org/10.1007/s10068-010-0025-y

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  • DOI: https://doi.org/10.1007/s10068-010-0025-y

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