Abstract
Paraoxonase-1 (PON1) is involved in the oxidative stress process that cause tissue damage observed in systemic lupus erythematosus (SLE) and anti-phospholipid syndrome (APS). The aim of the present study was to investigate the association of PON1 Q192R and L55M polymorphisms with risk of SLE and associated APS among Egyptian sample. The study included 120 SLE patients (45 without APS and 75 with APS) and 120 healthy subjects. PON1 Q192R and L55M polymorphisms were genotyped by real-time PCR. No significant differences in Q192R genotypes or allele frequencies were found between patients and controls (p = 0.5 and 0.1, respectively). The frequency of the 55M allele was significantly higher in SLE patients than in controls (66.6 vs. 43.3%), while the 55L allele was more frequent in controls (56.6%) than in patients (33.3%) (p = 0.03). The LL genotype was more frequent in controls (21.6%) than in patients (10%) while M allele carrier genotypes (LM + MM) were more frequent among patients (90%) than controls (78.3%), p = 0.04. Also, the 55M allele was more frequent in APS patients (73.3%) than in patients without APS (55.6%), p = 0.004. M allele carrier genotypes (LM + MM) was significantly higher among APS patients (95.4%) than in non-APS patients (80%), p = 0.008. Our results indicated that the PON1 L55M polymorphism associated with SLE and associated APS in a population from Cairo of Egypt, while the Q192R polymorphism plays no role in disease susceptibility. A large scale study to assess PON1 polymorphisms, PON1 activity, and markers of oxidative stress interaction is needed to clarify the role of PON-1 polymorphisms in the pathogenesis of SLE and associated APS.
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References
Dasgupta S, Demirci Y, Dressen A, Kao A, Rhew E, Goldman R, Manzi S, Kammerer C, Kamboh I (2011) Association analysis of PON2 genetic variants with serum paraoxonase activity and systemic lupus erythematosus. BMC Med Genet 12:7
Bahrehmand F, Vaisi-Raygani A, Ahmadi R, Kiani A, Rahimi Z, Tavilani H, Pourmotabbed T (2013) Paraoxonase (PON1) 55 polymorphism and association with systemic lupus erythematosus. Iran J Allergy Asthma Immunol 12(3):211–219
Passam FH, Giannakopoulos B, Mirarabshahi P, Krilis SA (2011) Molecular pathophysiology of the antiphospholipid syndrome: the role of oxidative post-translational modification of beta 2 glycoprotein I. Thromb Haemost 9(Suppl 1):275–282
Lai ZW, Marchena-Mendez I, Perl A (2015) Oxidative stress and Treg depletion in lupus patients with anti-phospholipid syndrome. Clin Immunol 158:148–152
Yang J, Yang X, Zou H, Li M (2016) Oxidative stress and Treg and Th17 dysfunction in systemic lupus erythematosus. Oxidative Med Cell Longev 2016:2526174
Shahmohamadnejad S, Vaisi-Raygani A, Shakiba Y, Kiani A, Rahimi Z, Bahrehmand F, Shakiba E, Pourmotabbed T (2015) Association between butyrylcholinesterase activity and phenotypes, paraoxonase192 rs662 gene polymorphism and their enzymatic activity with severity of rheumatoid arthritis: correlation with systemic inflammatory markers and oxidative stress, preliminary report. Clin Biochem 48(1–2):63–69
Elfasakhany F, Abou-Elnoeman S, Hussein M, Lamfoon H, Beyari M (2014) Paraoxonase activity and gene polymorphism in rheumatoid arthritis among Egyptians. Clinical Medicine and Diagnostics 4(1A):15–20
Hochberg MC (1997) Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arth Rheum 40:1725
Petri M, Orbai AM, Alarcón GS, Gordon C, Merrill JT, Fortin PR, Bruce IN, Isenberg D, Wallace DJ, Nived O, Sturfelt G, Ramsey-Goldman R, Bae SC, Hanly JG, Sánchez-Guerrero J, Clarke A, Aranow C, Manzi S, Urowitz M, Gladman D, Kalunian K, Costner M, Werth VP, Zoma A, Bernatsky S, Ruiz-Irastorza G, Khamashta MA, Jacobsen S, Buyon JP, Maddison P, Dooley MA, van Vollenhoven RF, Ginzler E, Stoll T, Peschken C, Jorizzo JL, Callen JP, Lim SS, Fessler BJ, Inanc M, Kamen DL, Rahman A, Steinsson K, Franks AG Jr, Sigler L, Hameed S, Fang H, Pham N, Brey R, Weisman MH, McGwin G Jr, Magder LS (2012) Derivation and validation of the systemic lupus international collaborating clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum 64(8):2677–2686
Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R, Derksen RH, DE Groot PG, Koike T, Meroni PL, Reber G, Shoenfeld Y, Tincani A, Vlachoyiannopoulos PG, Krilis SA (2006) International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS) [see comment] [136 refs]. J Thromb Haemost 4:295–306
Bombardier C, Gladman DD, Urowitz MB, Caron D, Chang CH (1992) Derivation of the SLEDAI. A disease activity index for lupus patients. The Committee on Prognosis Studies in SLE. Arthritis Rheum 35:630–640
Franco JS, Molano-González N, Rodríguez-Jiménez M, Acosta-Ampudia Y, Mantilla RD, Amaya-Amaya J, Rojas-Villarraga A, Anaya JM (2014) The coexistence of antiphospholipid syndrome and systemic lupus erythematosus in Colombians. PLoS One 9(10):e110242
López-Pedrera C, Barbarroja N, Jimenez-Gomez Y, Collantes-Estevez E, Aguirre MA, Cuadrado MJ (2016) Oxidative stress in the pathogenesis of atherothrombosis associated with anti-phospholipid syndrome and systemic lupus erythematosus: new therapeutic approaches. Rheumatology (Oxford)
Tripi LM, Manzi S, Chen Q, Kenney M, Shaw P, Kao A, Bontempo F, Kammerer C, Kamboh MI (2006) Relationship of serum paraoxonase 1 activity and paraoxonase 1 genotype to risk of systemic lupus erythematosus. Arthritis Rheum 54(6):1928–1939
Hashemi M, Moazeni-Roodi A, Fazaeli A, Sandoughi M, Bardestani GR, Kordi-Tamandani DM, Ghavami S (2010) Lack of association between paraoxonase-1 Q192R polymorphism and rheumatoid arthritis in southeast Iran. GenetMolRes (1):333–339
Kaman D, Ilhan N, Metin K, Akbulut M, Ustundag B (2009) A preliminary study of human paraoxonase and PON 1L/M55-PON 1 Q/R 192 polymorphisms in Turkish patients with coronary artery disease. Cell Biochem Funct 27:88–92
Ali OM, Effat LK, Amr KS, Azeem AA, Hassan SM (2014) Contribution of PON1 polymorphism in senile cataract among diabetic and non-diabetic Egyptian patients. J Mol Biomark Diagn 5:180. doi:10.4172/2155-9929.1000180
Fekih O, Triki S, Rejeb J, Neffati F, Douki W, Ommezzine A, Chouchane S, Guediche MN, Bouslama A, Najjar MF (2016) Paraoxonase 1 polymorphisms (L55M and Q192R) as a genetic marker of diabetic nephropathy in youth with type 1 diabetes. Endokrynol Pol. doi:10.5603/EP.a2016.0027
Gorshunskaia MI, Karachentsev II, Atramentova LA, Tyzhnenko TV, Kravchun NA, Pocherniaev AK, Poltorak VV (2011) Q192R polymorphism of PON-1 gene in type 2 diabetic patients. Tsitol Genet 45(1):48–51
Yilmaz A, Emre S, Agachan B, Bilge I, Yilmaz H, Ergen A, Isbir T, Sirin A (2009) Effect of paraoxonase 1 gene polymorphisms on clinical course of Henoch-Schönlein purpura. J Nephrol 22(6):726–732
Rahman MF, Hashad IM, Abou-Aisha K, Abdel-Maksoud SM, Gad MZ (2015) Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction. Arch Med Sci 11(3):513–520. doi:10.5114/aoms.2015.52353
El-Lebedy D, Kafoury M, Abd-El Haleem D, Ibrahim A, Awadallah E, Ashmawy I (2014) Paraoxonase-1 gene Q192R and L55M polymorphisms and risk of cardiovascular disease in Egyptian patients with type 2 diabetes mellitus. J Diabetes Metab Disord 13:124
Vaisi-Raygani A, Ghaneialvar H, Rahimi Z, Tavilani H, Pourmotabbed T, Shakiba E, Vaisi-Raygani A, Kiani A, Aminian M, Alibakhshi R, Bartels C (2011) Paraoxonase Arg 192 allele is an independent risk factor for three-vessel stenosis of coronary artery disease. Mol Biol Rep 38(8):5421–5428
Gürbüz Ş, Yıldız M, Kara M, Kargün K, Gürger M, Ateşçelik M, Alataş ÖD (2015) Paraoxonase-1 gene in patients with chronic obstructive pulmonary disease investigation Q192R and L55M polymorphisms. World J Emerg Med 6(3):201–206. doi:10.5847/wjem.j.1920-8642.2015.03.007
Ortak H, Söğüt E, Ateş O, Erkorkmaz U, Benli I, Akbas A, Demir S, Ozyurt H (2013) Protective effect of paraoxonase 1 gene variant L55M in retinal vein occlusion. Mol Vis 19:486–xxx
Hussein YM, Gharib AF, Etewa RL, ElSawy WH (2011) Association of L55M and Q192R polymorphisms in paraoxonase 1 (PON1) gene with breast cancer risk and their clinical significance. Mol Cell Biochem 351(1–2):117–123. doi:10.1007/s11010-011-0718-4 Epub 2011 Jan 13
Asefi M, Vaisi-Raygani A, Bahrehmand F, Kiani A, Rahimi Z, Nomani H, Ebrahimi A, Tavilani H, Pourmotabbed T (2012) Paraoxonase 1 (PON1) 55 polymorphism, lipid profiles and psoriasis. Br J Dermatol 167(6):1279–1286
Bahrehmand F, Vaisi-Raygani A, Rahimi Z, Ahmadi R, Kiani A, Tavilani H, Vaisi-Raygani H, Pourmotabbed T (2014) Synergistic effects of BuChE non-UU phenotype and paraoxonase (PON1) 55 M allele on the risk of systemic lupus erythematosus: influence on lipid and lipoprotein metabolism and oxidative stress, preliminary report. Lupus 23(3):263–272
Sayın Kocakap DB, Doğru MT, Şimşek V, Çabuk F, Yıldırım N, Çelik Y, Alyılmaz Bekmez S, Erdem S (2015) The association of paraoxonase 1 gene L55M polymorphism with the extent and severity of coronary artery disease in the Turkish population and its dependence on gender. Anatol J Cardiol. doi:10.5152/akd.2015.6010
Küçükali CI, Ulusoy C, Özkan Ö, Orhan N, Güleç H, Erdağ E, Buker S, Tüzün E (2015) Evaluation of paraoxonase 1 polymorphisms in patients with bipolar disorder. In Vivo 29(1):103–108
Lakshmy R, Ahmad D, Abraham R, Sharma M, Vemparala K, Siuli Das K, Reddy S, Prabhakaran D (2010) Paraoxonase gene Q192R & L55M polymorphisms in Indians with acute myocardial infarction & association with oxidized low density lipoprotein. Indian J Med Res 131:522–529
Zakrzewska-Pniewska B, Nojszewska M, Róg T, Pniewski J, Dorobek M, Styczyńska M, Szczudlik A (2013) Polymorphisms of paraoxonase 1 and 2 genes and the risk of multiple sclerosis in the Polish population. Neurol Neurochir Pol 47(1):49–52
Chen Y, Huang R, Chen K, Song W, Yang Y, Zhao B, Li J, Shang HF (2012) Association analysis of PON polymorphisms in sporadic ALS in a Chinese population. Neurobiol Aging 33(12):2949 e1-3
Liu YL, Yang J, Zheng J, Liu DW, Liu T, Wang JM, Wang CN, Wang MW, Tian QB (2012) Paraoxonase 1 polymorphisms L55M and Q192R were not risk factors for Parkinson’s disease: a HuGE review and meta-analysis. Gene 501(2):188–192
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Ibrahim, A.A., El-Lebedy, D., Ashmawy, I. et al. Association between paraoxonase-1 gene Q192R and L55M polymorphisms in systemic lupus erythematosus (SLE) and anti-phospholipid syndrome (APS) in a population from Cairo of Egypt. Clin Rheumatol 36, 1305–1310 (2017). https://doi.org/10.1007/s10067-017-3567-z
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DOI: https://doi.org/10.1007/s10067-017-3567-z