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Plasma glutathione peroxidase (GSH-Px) concentration is elevated in rheumatoid arthritis: a case–control study

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Abstract

Plasma glutathione peroxidase (GSH-Px) by enzyme-linked immunosorbent assay (ELISA) offers a complimentary measurement approach to traditional GSH-Px activity methods. The aim was to investigate whether GSH-Px measured by ELISA in rheumatoid arthritis patients was elevated compared to controls. This was a case–control study with rheumatoid arthritis patients recruited from private practice and gender and age-matched controls randomly selected from the electoral role. GSH-Px concentration was measured by ELISA. Plasma malondialdehyde was used as a measure of oxidative stress, and antioxidant capacity was measured based on reduction of Cu++ to Cu+ by antioxidants in the sample. Disease severity was measured using the Health Assessment Questionnaire-Disability Index (HAQ-DI) and C-reactive protein was measured using an immunoturbidometric method. A total of 74 patients were recruited, consisting of 35 rheumatoid arthritis cases and 39 healthy controls. There were no differences between rheumatoid arthritis cases and controls for oxidative stress and antioxidant capacity; however, GSH-Px concentration was markedly elevated in the rheumatoid arthritis sufferers (85.9 ± 147.7 versus 17.3 ± 13.0 mg/L, respectively; mean ± SD; p < 0.01). GSH-Px levels were not associated with severity measured by the HAQ-DI or C-reactive protein. Patients with rheumatoid arthritis demonstrated increased GSH-Px consistent with an adaptive upregulation of GSH-Px to protect against oxidative stress.

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Acknowledgments

The authors wish to thank Catrina Boon for assistance with the provision of stored samples. This work was supported by the School of Pharmacy as part of a student project. TASOAC was funded by NHMRC and Arthritis Australia.

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Authors and Affiliations

  1. School of Pharmacy, University of Tasmania, Private Bag 26, GPO Box 252, Hobart, Tasmania, 7001, Australia

    Glenn A. Jacobson, Stephen J. Ives & Christian Narkowicz

  2. Menzies Research Institute Tasmania, University of Tasmania, Hobart, Tasmania, Australia

    Graeme Jones

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  1. Glenn A. Jacobson
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  2. Stephen J. Ives
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  3. Christian Narkowicz
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  4. Graeme Jones
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Correspondence to Glenn A. Jacobson.

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Jacobson, G.A., Ives, S.J., Narkowicz, C. et al. Plasma glutathione peroxidase (GSH-Px) concentration is elevated in rheumatoid arthritis: a case–control study. Clin Rheumatol 31, 1543–1547 (2012). https://doi.org/10.1007/s10067-012-2046-9

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  • DOI: https://doi.org/10.1007/s10067-012-2046-9

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