Abstract
Nestin is an intermediate filament protein expressed in undifferentiated cells during central nervous system development, and glioma is known to be a highly infiltrative tumor. We determined whether nestin was expressed in astrocytic tumors and could identify infiltrating tumor cells. We screened 65 archival, paraffin-embedded adult astrocytic tumors using immunohistochemical staining and computerized overlaid photographs. Normal biopsied brains and metastatic brain tumors were also examined. The intensity of nestin expression corresponded to the tumor grade. All 33 glioblastoma cases showed positive and extensive staining, which was less positive in diffuse astrocytoma. Overlaid images showed that nestin immunostaining delineated tumor invasion into adjacent gray and white matter. Nestin is a useful marker for examining the infiltration of malignant cells into surrounding tissue.
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Duggal N, Hammond RR (2002) Nestin expression in ganglioglioma. Exp Neurol 174:89–95
Hockfield S, McKay RDG (1985) Identification of major cell classes in the developing mammalian nervous system. J Neurosci 5:3310–3328
Tohyama T, Lee VM-Y, Rorke LB, et al (1992) Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Lab Invest 66:303–313
Dahlstrand J, Collins VP, Lendahl U (1992) Expression of the class VI intermediate filament nestin in human central nervous system tumors. Cancer Res 52:5334–5341
Florenes VA, Holm R, Myklebost O, et al (1994) Expression of the neuroectodermal intermediate filament nestin in human melanomas. Cancer Res 54:354–356
Kobayashi M, Sjoberg G, Soderhall S, et al (1998) Pediatric rhabdomyosarcomas express the intermediate filament nestin. Pediatr Res 43:386–392
Almqvist PM, Mah R, Lendahl U, et al (2002) Immunohistochemical detection of nestin in pediatric brain tumors. J Histochem Cytochem 50:147–158
Duggal N, Iskander S, Hammond RR (2003) MAP2 and nestin co-expression in dysembryoplastic neuroepithelial tumors. Clin Neuropathol 22:57–65
Louis DN, Ohgaki H, Wiestler OD, et al (2007) WHO classification of tumours of the central nervous system. Pathology and genetics of tumors of the nervous system. International Agency for Research on Cancer, World Health Organization, Lyon, pp 13-52
Takano T, Becker LE (1997) Developmental change of the nestinimmunoreactive midline raphe glial structure in human brainstem and spinal cord. Dev Neurosci 19:202–209
Johansson CB, Momma S, Clarke DL, et al (1999) Identification of a neural stem cell in the adult mammalian central nervous system. Cell 96:25–34
Frisen J, Johansson CB, Torok C, et al (1995) Rapid, widespread, and long-lasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. J Cell Biol 131:453–464
Krum JM, Rosenstein JM (1999) Transient coexpression of nestin, GFAP, and vascular endothelial growth factor in mature reactive astroglia following neural grafting or brain wounds. Exp Neurol 160:348–360
Li Y, Chopp M (1999) Temporal profile of nestin expression after focal cerebral ischemia in adult rat. Brain Res 838:1–10
Lin RC, Matesic DF, Marvin M, et al (1995) Re-expression of the intermediate filament nestin in reactive astrocytes. Neurobiol Dis 2:79–85
Ehrmann J, Kolar Z, Mokry J (2005) Nestin as a diagnostic and prognostic marker: immunohistochemical analysis of its expression in different tumors. J Clin Pathol 58:222–223
Sugawara K, Kurihara H, Negishi M, et al (2002) Nestin as a marker for proliferative endothelium in gliomas. Lab Invest 82:345–351
Suzuki SO, Kitai R, Llena J, et al (2002) MAP-2e, a novel MAP-2 isoform, is expressed in gliomas and delineates tumor architecture and patterns of infiltration. J Neuropathol Exp Neurol 61:403–412
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Kitai, R., Horita, R., Sato, K. et al. Nestin expression in astrocytic tumors delineates tumor infiltration. Brain Tumor Pathol 27, 17–21 (2010). https://doi.org/10.1007/s10014-009-0261-0
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DOI: https://doi.org/10.1007/s10014-009-0261-0