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High-throughput nucleotide sequencing for bacteriome studies in oral squamous cell carcinoma: a systematic review

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Abstract

Purpose

Dysbiosis has been identified in oral squamous cell carcinoma (OSCC). The aim of this study was to carry out a systematic review of an electronic research that was carried out on articles published between January 2008 and September 2018.

Methods

Eight studies were selected after applying the inclusion and exclusion criteria.

Results

All articles targeted the hypervariable regions of the 16S rRNA gene. At the phylum level, it was found reduction of Bacteroidetes (2/8 studies) and increase of Firmicutes (2/8 studies). At the genus level, Rothia increased (1/8 studies) and decreased (2/8 studies) in tumor samples, and Streptococcus also was found increased (3/8 studies) and reduced (3/8 studies). Fusobacterium only increased in OSCC samples (3/8 studies). At species level, an increase in F. nucleatum subsp. polymorphum was more associated to OSCC (2/8 studies) than with controls, as was P. aeruginosa (3/8 studies).

Conclusion

In summary, the results corroborated dysbiosis in OSCC patients, with enrichment of microbial taxa that are associated with inflammation and production of acetaldehyde. However, variations of study design and sample size were observed among the studies, as well as a shortage of more detailed analyses of possible correlations between risk habits and OSCC. This lack of more detailed analysis may be the cause of the inconsistencies in regard of the alterations reported for certain genera and species. In conclusion, there is an association between OSCC and oral microbiota dysbiosis, but its role in oral carcinogenesis needs to be clarified in more detail.

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Funding

This work was supported by the National Council for Scientific and Technological Development (CNPq) (Project: 211309/2013-3) and the Foundation for Research Financial Support in the State of Rio de Janeiro (FAPERJ), Rio de Janeiro, Brazil (Project: E26/103.001/2012).

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Author notes

  1. Ruth Tramontani Ramos, Camila Stofella Sodré, Henrique Fragoso dos Santos, Lucio Souza Goncalves and Dennis de Carvalho Ferreira contributed equally to this work.

Authors and Affiliations

  1. Department of Medical Clinic, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

    Ruth Tramontani Ramos & Camila Stofella Sodré

  2. Faculty of Dentistry, Estácio de Sá University (UNESA), Rio de Janeiro, Brazil

    Paulo Matheus Guerra Ribeiro de Sousa Rodrigues, Alexandre Marques Paes da Silva, Lucio Souza Gonçalves & Dennis de Carvalho Ferreira

  3. Faculty of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

    Milenna Silva Fuly

  4. Department of Marine Biology, Fluminense Federal University (UFF), Rio de Janeiro, Brazil

    Henrique Fragoso dos Santos

  5. Service of Medical Genetics, IPPMG - Martagão Gesteira Pediatric Institute, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

    Marcia Gonçalves Ribeiro

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  1. Ruth Tramontani Ramos
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Correspondence to Lucio Souza Gonçalves.

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Ramos, R.T., Sodré, C.S., de Sousa Rodrigues, P.M.G.R. et al. High-throughput nucleotide sequencing for bacteriome studies in oral squamous cell carcinoma: a systematic review. Oral Maxillofac Surg 24, 387–401 (2020). https://doi.org/10.1007/s10006-020-00873-4

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