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Simultaneous demonstration of gelatinolytic activity, morphology, and immunohistochemical reaction using zymography film

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Abstract

In situ zymography has been used to assess gelatinolytic activity, which is mainly due to matrix metalloproteinases (MMPs) in cancer tissues. MMPs play an important role in cancer invasion and metastasis. Film in situ zymography (FIZ) enables the in situ evaluation of gelatinolytic activity with high reproducibility. In this article, we report a study of FIZ, in a case of breast cancer with an invasive carcinoma component showing clear gelatinolytic activity, and in a non-invasive carcinoma component showing little gelatinolytic activity. Immunohistochemistry on FIZ was also performed. The simultaneous detection of gelatinolytic activity and immunohistochemical reaction was established in a single film. Immunohistochemistry on FIZ may have good potential for the investigation of cancer microenvironment.

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Authors and Affiliations

  1. Department of Pathology, Kawasaki Medical School, Matsushima 577, Kurashiki, Okayama, 701-0192, Japan

    Naoki Kanomata & Takuya Moriya

  2. Pathology Division, National Cancer Center Research Institute East, Kashiwa, Japan

    Naoki Kanomata, Takahiro Hasebe & Atsushi Ochiai

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  1. Naoki Kanomata
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  2. Takahiro Hasebe
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  3. Takuya Moriya
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  4. Atsushi Ochiai
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Correspondence to Naoki Kanomata.

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Kanomata, N., Hasebe, T., Moriya, T. et al. Simultaneous demonstration of gelatinolytic activity, morphology, and immunohistochemical reaction using zymography film. Med Mol Morphol 46, 193–197 (2013). https://doi.org/10.1007/s00795-013-0021-4

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  • DOI: https://doi.org/10.1007/s00795-013-0021-4

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