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Morphology and infectivity of virus that persistently caused infection in an AGS cell line

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Abstract

A recent report has indicated that proteins and genes of simian virus 5 (SV5) are detected in a human gastric adenocarcinoma (AGS) cell line, which is widely provided for oncology, immunology, and microbiology research. However, the production of infective virions has not been determined in this cell line. In this study, the morphology and infectivity of the virus particles of the AGS cell line were studied by light and electron microscopy and virus transmission assay. The virus particles were approximately 176.0 ± 41.1 nm in diameter. The particles possessed projections 8–12 nm long on the surface and contained a nucleocapsid determined to be 13–18 nm in width and less than 1,000 nm in length. The virus was transmissible to the Vero cell line, induced multinuclear giant cell formation, and reproduced the same shape of antigenic virions. In this study, the persistently infected virus in the AGS cell line was determined to be infective and form reproducible virions, and a new morphological feature of SV5 was determined.

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Authors and Affiliations

  1. Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan

    Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno & Kouichi Sano

  2. National Institute of Infectious Diseases, Tokyo, Japan

    Tomohiko Takasaki

Authors
  1. Yukimasa Ooi
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  2. Eriko Daikoku
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  3. Hong Wu
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  4. Hiroaki Aoki
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  5. Chizuko Morita
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  6. Takashi Nakano
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  7. Takehiro Kohno
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  8. Tomohiko Takasaki
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  9. Kouichi Sano
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Correspondence to Kouichi Sano.

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Ooi, Y., Daikoku, E., Wu, H. et al. Morphology and infectivity of virus that persistently caused infection in an AGS cell line. Med Mol Morphol 44, 213–220 (2011). https://doi.org/10.1007/s00795-010-0530-3

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  • DOI: https://doi.org/10.1007/s00795-010-0530-3

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