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Immunohistochemical and electron microscopic characterization of brush cells of the rat cecum

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Abstract

Brush cells (BCs) are relatively rare cells that are sparsely distributed throughout the mammalian digestive and respiratory systems. BCs have been identified in the rodent large intestine, but these cells have not been characterized by immunocytochemistry or electron microscopy. We previously demonstrated that rat bile duct BCs had strong immunoreactivity for six proteins that function in HCO −3 secretion and thus assumed that BCs secrete NaHCO3. It is well known that the gastrointestinal (GI) tract secretes NaHCO3, but it is not known whether BCs of the GI tract also express proteins related to HCO −3 secretion. Thus, in the present study, using double immunostaining for cytokeratin 18, a specific marker for BCs, we investigated protein expression in BCs from the rodent GI tract. We show that BCs from the GI tract express six proteins related to HCO −3 secretion: cystic fibrosis transmembrane conductance regulator (CFTR), Cl−/HCO −3 exchanger, Na+/HCO −3 cotransporter, carbonic anhydrase II, Na+/H+ exchanger (NHE) 1, and NHE3. These results suggest that BCs from the GI tract secrete NaHCO3. In addition, we examined BCs from the rat cecum using electron microscopy (EM). Transmission EM (TEM) showed that BCs have long microvilli, a well-developed tubulovesicular system, and an abundant cytoskeleton. Scanning EM revealed that BCs were scattered on the luminal surface of the cecum and had numerous long microvilli.

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References

  1. Rhodin JAG, Dalhamn T (1956) Electron microscopy of the tracheal ciliated mucosa in rat. Z Zellforsch 44:345–412

    Article  PubMed  CAS  Google Scholar 

  2. Ogata T (2000) Mammalian tuft (brush) cells and chloride cells of other vertebrate share a similar structure and cytochemical reactivities. Acta Histochem Cytochem 33:439–449

    Article  CAS  Google Scholar 

  3. Sbarbati A, Osculati F (2005) A new fate for old cells: brush cells and related elements. J Anat 206:349–358

    Article  PubMed  CAS  Google Scholar 

  4. Sato A (2007) Tuft cells. Anat Sci Int 82f:187–199

    Article  Google Scholar 

  5. Isomäki AM (1973) A new cell type (tuft cell) in the gastrointestinal mucosa of the rat. Acta Pathol Microbiol Scand Sect A (Suppl) 240:1–35

    Google Scholar 

  6. Nabeyama A, Leblond CP (1974) “Caveolated cells” characterized by deep surface invaginations and abundant filaments in mouse gastro-intestinal epithelia. Am J Anat 140:147–166

    Article  PubMed  CAS  Google Scholar 

  7. Sato A, Miyoshi S (1997) Fine structure of tuft cells of the main excretory duct epithelium in the rat submandibular gland. Anat Rec 248:325–331

    Article  PubMed  CAS  Google Scholar 

  8. Luciano L, Groos S, Reale E (2003) Brush cells of rodent gallbladder and stomach epithelia express neurofilaments. J Histochem Cytochem 51:187–198

    PubMed  CAS  Google Scholar 

  9. Hijiya K (1978) Electron microscope study of the alveolar brush cell. J Electron Microsc 27:223–227

    CAS  Google Scholar 

  10. Höfer D, Drenckhahn D (1998) Identification of the taste cell Gprotein, α-gustducin, in brush cells of the rat pancreatic duct system. Histochem Cell Biol 110:303–309

    Article  PubMed  Google Scholar 

  11. Ogata T (2005) Sodium bicarbonate secretion indicated by ultrastructural cytochemical localization of HCO −3 , Cl−, and Na+ ions on rat bile duct brush cells. Med Mol Morphol 38:243–250

    Article  PubMed  CAS  Google Scholar 

  12. Ogata T (2006) Bicarbonate secretion by rat bile duct brush cells indicated by immunohistochemical localization of CFTR, anion exchanger AE2, Na+/HCO −3 cotransporter, carbonic anhydrase II, Na+/H+ exchangers NHE1 and NHE3, H+/K+-ATPase, and Na+/K+-ATPase. Med Mol Morphol 39:44–48

    Article  PubMed  CAS  Google Scholar 

  13. Argent BE, Case RM (1994) Pancreatic ducts. Cellular mechanism and control of bicarbonate secretion. In: Johnson LR (ed) Physiology of the gastrointestinal tract, 3rd edn. Raven Press, New York, pp 1473–1497

    Google Scholar 

  14. Marino CR, Gorelick FS (2003) Pancreatic and salivary glands. In: Boron WF, Boulpaep EL (eds) Medical physiology. Saunders, Philadelphia, pp 908–930

    Google Scholar 

  15. Steward MC, Ishiguro H, Case RM (2005) Mechanisms of bicarbonate secretion in the pancreatic duct. Annu Rev Physiol 67:377–409

    Article  PubMed  CAS  Google Scholar 

  16. Höfer D, Drenckhahn D (1996) Cytoskeletal markers allowing discrimination between brush cells and other epithelial cells of the gut including enteroendocrine cells. Histochem Cell Biol 105:405–412

    Article  PubMed  Google Scholar 

  17. Flemström G, Garner A (1982) Gastroduodenal HCO −3 transport: characteristics and proposed role in acidity regulation and mucosal protection. Am J Physiol 242:G183–G193

    PubMed  Google Scholar 

  18. Kawamata K, Hayashi H, Suzuki Y (2006) Chloride-dependent bicarbonate secretion in the mouse large intestine. Biomed Res 27:15–21

    Article  PubMed  CAS  Google Scholar 

  19. Hatta S, Sakamoto J, Horio Y (2002) Ion channels and diseases. Med Electron Microsc 35:117–126

    Article  PubMed  CAS  Google Scholar 

  20. Roussa E, Romero MF, Schmitt BM, Boron WF, Alper SL, Thévenod F (1999) Immunolocalization of anion exchanger AE2 and Na+-HCO −3 cotransporter in rat parotid and submandibular glands. Am J Physiol 277:G1288–G1296

    PubMed  CAS  Google Scholar 

  21. Roussa E, Alper SL, Thévenod F (2001) Immunolocalization of anion exchanger AE2, Na+/H+ exchangers NHE1 and NHE4, and vacuolar type H+-ATPase in rat pancreas. J Histochem Cytochem 49:463–474

    PubMed  CAS  Google Scholar 

  22. Marino CR, Jeanes V, Boron WF, Schmitt BM (1999) Expression and distribution of the Na+-HCO −3 cotransporter in human pancreas. Am J Physiol 277:G487–G494

    PubMed  CAS  Google Scholar 

  23. Thévenod F, Roussa E, Schmitt BM, Romero MF (1999) Cloning and immunolocalization of a rat pancreatic Na+ bicar bonate cotransporter. Biochem Biophys Res Commun 264: 291–298

    Article  PubMed  Google Scholar 

  24. Satoh H, Moriyama N, Hara C, Yamada H, Horita S, Kunimi M, Tsukamoto K, Iso-o N, Inatomi J, Kawakami H, Kudo A, Endou H, Igarashi T, Goto A, Fujita T, Seki G (2003) Localization of Na+-HCO −3 cotransporter (NBC-1) variants in rat and human pancreas. Am J Physiol 284:C729–C737

    CAS  Google Scholar 

  25. Kumpulainen T, Jalovaara P (1981) Immunohistochemical localization of carbonic anhydrase isoenzymes in the human pancreas. Gastroenterology 80:796–799

    PubMed  CAS  Google Scholar 

  26. Parkkila S, Parkkila AK, Juvonen T, Rajaniemi H (1994) Distribution of the carbonic anhydrase isoenzymes I, II, and VI in the human alimentary tract. Gut 35:645–650

    Article  Google Scholar 

  27. Spicer SS, Ge ZH, Tashian RE, Hazen-Martin DJ, Schulte BA (1990) Comparative distribution of carbonic anhydrase isozymes III and II in rodent tissues. Am J Anat 187:55–64

    Article  PubMed  CAS  Google Scholar 

  28. Wattel W, Geuze JJ (1978) The cells of the rat gastric groove and cardia. An ultrastructural and carbohydrate histochemical study, with special reference to the fibrillovesicular cells. Cell Tissue Res 186:375–391

    CAS  Google Scholar 

  29. Luciano L, Reale E, Ruska H (1968) Über eine glykogenhaltige Bürstenzelle in Rectum der Ratte. Z Zellforsh 91:153–158

    Article  CAS  Google Scholar 

  30. Sato A, Miyoshi S (1988) Ultrastructure of the main excretory duct epithelia of the rat parotid and submandibular glands with a review of the literature. Anat Rec 220:239–251

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, Kochi Medical School, Oko-cho, Nankoku, Kochi, 783-8505, Japan

    Ken Okamoto, Kazuhiro Hanazaki, Toyokazu Akimori, Takehiro Okabayashi & Takuro Ogata

  2. Department of Anatomy, Kochi Medical School, Kochi, Japan

    Teruhiko Okada

  3. Department of Human Health and Medical Services, Kochi Medical School, Kochi, Japan

    Michiya Kobayashi

  4. Department of Physiology, Kochi Medical School, Kochi, Japan

    Takuro Ogata

  5. Central Medical Laboratory, Kochi Medical School, Kochi, Japan

    Takuro Ogata

Authors
  1. Ken Okamoto
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  2. Kazuhiro Hanazaki
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  3. Toyokazu Akimori
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  4. Takehiro Okabayashi
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  5. Teruhiko Okada
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  6. Michiya Kobayashi
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  7. Takuro Ogata
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Correspondence to Kazuhiro Hanazaki.

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Okamoto, K., Hanazaki, K., Akimori, T. et al. Immunohistochemical and electron microscopic characterization of brush cells of the rat cecum. Med Mol Morphol 41, 145–150 (2008). https://doi.org/10.1007/s00795-008-0412-0

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  • DOI: https://doi.org/10.1007/s00795-008-0412-0

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