Abstract
Four extremely halophilic archaeal strains (ZJ2T, BND6T, DT87T, and YPL30T) were isolated from marine environments and a salt mine in China. The 16S rRNA and rpoB′ gene sequence similarities among strains ZJ2T, BND6T, DT87T, YPL30T and the current species of Natrinema were 93.2–99.3% and 89.2–95.8%, respectively. Both phylogenetic and phylogenomic analyses revealed that strains ZJ2T, BND6T, DT87T, and YPL30T cluster with the Natrinema members. The overall genome-related indexes (ANI, isDDH, and AAI) among these four strains and the current species of genus Natrinema were 70–88%, 22–43% and 75–89%, respectively, clearly below the threshold values for species boundary. Strains ZJ2T, BND6T, DT87T, and YPL30T could be distinguished from the related species according to differential phenotypic characteristics. The major polar lipids of the four strains were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and disulfated mannosyl glucosyl diether (S2-DGD). The phenotypic, chemotaxonomic, phylogenetic and phylogenomic features indicated that strains ZJ2T (= CGMCC 1.18786 T = JCM 34918 T), BND6T (= CGMCC 1.18777 T = JCM 34909 T), DT87T (= CGMCC 1.18921 T = JCM 35420 T), and YPL30T (= CGMCC 1.15337 T = JCM 31113 T) represent four novel species of the genus Natrinema, for which the names, Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., and Natrinema zhouii sp. nov., are proposed.
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Data Availability
The sequences determined in this study were deposited and available in the NCBI GenBank.
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Acknowledgements
We are grateful to Dr. Bao-Zhu Fang (Sun Yat-Sen University) for sampling marine sediment, Dr. Shao-Xing Chen (Anhui Normal University) for kindly providing salt mine saline soil, and Dr. De-Chao Zhang (Institute of Oceanology, Chinese Academy of Sciences) for sampling coastal saline soil. The current authors are indebted to the graduate students graduated (Chen-Xi Bao, Yu-Jie Xin, Pan-Pan Yuan) for their primary contribution to this work.
Funding
This work was financially supported by the National Natural Science Foundation of China (No. 32070003), and the National Science and Technology Fundamental Resources Investigation Program of China (No. 2019FY100700; No. 2021FY100900).
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Hu, Y., Ma, X., Li, XX. et al. Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., Natrinema zhouii sp. nov., extremely halophilic archaea isolated from marine environments and a salt mine. Extremophiles 27, 9 (2023). https://doi.org/10.1007/s00792-023-01294-3
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DOI: https://doi.org/10.1007/s00792-023-01294-3